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  Decoding Human Cytomegalovirus

Stern-Ginossar, N., Weisburd, B., Michalski, A., Vu Khanh Le, T., Hein, M. Y., Huang, S.-X., et al. (2012). Decoding Human Cytomegalovirus. SCIENCE, 338(6110), 1088-1093. doi:10.1126/science.1227919.

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 Creators:
Stern-Ginossar, Noam1, Author
Weisburd, Ben1, Author
Michalski, Annette2, Author              
Vu Khanh Le, Thuy1, Author
Hein, Marco Y.2, Author              
Huang, Sheng-Xiong1, Author
Ma, Ming1, Author
Shen, Ben1, Author
Qian, Shu-Bing1, Author
Hengel, Hartmut1, Author
Mann, Matthias2, Author              
Ingolia, Nicholas T.1, Author
Weissman, Jonathan S.1, Author
Affiliations:
1external, ou_persistent22              
2Mann, Matthias / Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565159              

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Free keywords: MHC CLASS-I; TRANSCRIPTIONAL ACTIVATION; TRANSLATION INITIATION; PROTEIN; GENE; GENOME; DNA
 Abstract: The human cytomegalovirus (HCMV) genome was sequenced 20 years ago. However, like those of other complex viruses, our understanding of its protein coding potential is far from complete. We used ribosome profiling and transcript analysis to experimentally define the HCMV translation products and follow their temporal expression. We identified hundreds of previously unidentified open reading frames and confirmed a fraction by means of mass spectrometry. We found that regulated use of alternative transcript start sites plays a broad role in enabling tight temporal control of HCMV protein expression and allowing multiple distinct polypeptides to be generated from a single genomic locus. Our results reveal an unanticipated complexity to the HCMV coding capacity and illustrate the role of regulated changes in transcript start sites in generating this complexity.

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Language(s): eng - English
 Dates: 2012-11-23
 Publication Status: Published in print
 Pages: 6
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: ISI: 000311390600046
DOI: 10.1126/science.1227919
 Degree: -

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Title: SCIENCE
Source Genre: Journal
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Publ. Info: 1200 NEW YORK AVE, NW, WASHINGTON, DC 20005 USA : AMER ASSOC ADVANCEMENT SCIENCE
Pages: - Volume / Issue: 338 (6110) Sequence Number: - Start / End Page: 1088 - 1093 Identifier: ISSN: 0036-8075