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  Tissue inducible Lifeact expression allows visualization of actin dynamics in vivo and ex vivo

Schachtner, H., Li, A., Stevenson, D., Calaminus, S. D. J., Thomas, S. G., Watson, S. P., et al. (2012). Tissue inducible Lifeact expression allows visualization of actin dynamics in vivo and ex vivo. EUROPEAN JOURNAL OF CELL BIOLOGY, 91(11-12), 923-929. doi:10.1016/j.ejcb.2012.04.002.

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Schachtner, Hannah1, Author
Li, Ang1, Author
Stevenson, David1, Author
Calaminus, Simon D. J.1, Author
Thomas, Steven G.1, Author
Watson, Steve P.1, Author
Sixt, Michael1, Author              
Wedlich-Soldner, Roland2, Author              
Strathdee, Douglas1, Author
Machesky, Laura M.1, Author
Affiliations:
1external, ou_persistent22              
2Wedlich-Söldner, Roland / Cellular Dynamics and Cell Patterning, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565174              

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Free keywords: F-ACTIN; MELANOBLAST MIGRATION; CONTRACTILE RING; TRANSGENIC MICE; HPRT LOCUS; RAC1; RECOMBINATION; MOTILITY; REVEALS; LINEAGEActin dynamics; Cell migration; Cytokinesis; Motility; Cytoskeleton; Imaging;
 Abstract: We describe here the development and characterization of a conditionally inducible mouse model expressing Lifeact-GFP, a peptide that reports the dynamics of filamentous actin. We have used this model to study platelets, megakaryocytes and melanoblasts and we provide evidence that Lifeact-GFP is a useful reporter in these cell types ex vivo. In the case of platelets and megakaryocytes, these cells are not transfectable by traditional methods, so conditional activation of Lifeact allows the study of actin dynamics in these cells live. We studied melanoblasts in native skin explants from embryos, allowing the visualization of live actin dynamics during cytokinesis and migration. Our study revealed that melanoblasts lacking the small GTPase Rac1 show a delay in the formation of new pseudopodia following cytokinesis that accounts for the previously reported cytokinesis delay in these cells. Thus, through use of this mouse model, we were able to gain insights into the actin dynamics of cells that could only previously be studied using fixed specimens or following isolation from their native tissue environment. (C) 2012 Published by Elsevier GmbH.

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Language(s): eng - English
 Dates: 2012-11
 Publication Status: Published in print
 Pages: 7
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Degree: -

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Title: EUROPEAN JOURNAL OF CELL BIOLOGY
Source Genre: Journal
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Publ. Info: OFFICE JENA, P O BOX 100537, 07705 JENA, GERMANY : ELSEVIER GMBH, URBAN & FISCHER VERLAG
Pages: - Volume / Issue: 91 (11-12) Sequence Number: - Start / End Page: 923 - 929 Identifier: ISSN: 0171-9335