English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Mediator Phosphorylation Prevents Stress Response Transcription During Non-stress Conditions

Miller, C., Matic, I., Maier, K. C., Schwalb, B., Roether, S., Straesser, K., et al. (2012). Mediator Phosphorylation Prevents Stress Response Transcription During Non-stress Conditions. JOURNAL OF BIOLOGICAL CHEMISTRY, 287(53), 44017-44026. doi:10.1074/jbc.M112.430140.

Item is

Files

show Files

Locators

show

Creators

show
hide
 Creators:
Miller, Christian1, Author
Matic, Ivan2, Author              
Maier, Kerstin C.1, Author
Schwalb, Bjoern1, Author
Roether, Susanne1, Author
Straesser, Katja1, Author
Tresch, Achim1, Author
Mann, Matthias2, Author              
Cramer, Patrick1, Author
Affiliations:
1external, ou_persistent22              
2Mann, Matthias / Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565159              

Content

show
hide
Free keywords: POLYMERASE-II TRANSCRIPTION; MESSENGER-RNA STABILITY; SACCHAROMYCES-CEREVISIAE; IN-VIVO; GENE-EXPRESSION; QUANTITATIVE PROTEOMICS; MULTISTRESS RESPONSE; SIGNAL-TRANSDUCTION; FUNCTIONAL-ANALYSIS; MASS-SPECTROMETRY
 Abstract: The multiprotein complex Mediator is a coactivator of RNA polymerase (Pol) II transcription that is required for the regulated expression of protein-coding genes. Mediator serves as an end point of signaling pathways and regulates Pol II transcription, but the mechanisms it uses are not well understood. Here, we used mass spectrometry and dynamic transcriptome analysis to investigate a functional role of Mediator phosphorylation in gene expression. Affinity purification and mass spectrometry revealed that Mediator from the yeast Saccharomyces cerevisiae is phosphorylated at multiple sites of 17 of its 25 subunits. Mediator phosphorylation levels change upon an external stimulus set by exposure of cells to high salt concentrations. Phosphorylated sites in the Mediator tail subunit Med15 are required for suppression of stress-induced changes in gene expression under non-stress conditions. Thus dynamic and differential Mediator phosphorylation contributes to gene regulation in eukaryotic cells.

Details

show
hide
Language(s): eng - English
 Dates: 2012-12-28
 Publication Status: Published in print
 Pages: 10
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: ISI: 000312938600001
DOI: 10.1074/jbc.M112.430140
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: JOURNAL OF BIOLOGICAL CHEMISTRY
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3996 USA : AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
Pages: - Volume / Issue: 287 (53) Sequence Number: - Start / End Page: 44017 - 44026 Identifier: ISSN: 0021-9258