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  In vivo knockdown of Brachyury results in skeletal defects and urorectal malformations resembling caudal regression syndrome

Pennimpede, T., Proske, J., Koenig, A., Vidigal, J. A., Morkel, M., Bramsen, J. B., et al. (2012). In vivo knockdown of Brachyury results in skeletal defects and urorectal malformations resembling caudal regression syndrome. Developmental Biology, 372(1), 55-67. doi:10.1016/j.ydbio.2012.09.003.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-000E-B514-D Version Permalink: http://hdl.handle.net/11858/00-001M-0000-000E-B515-B
Genre: Journal Article

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 Creators:
Pennimpede, Tracie1, Author              
Proske, Judith, Author
Koenig, Andrea1, Author              
Vidigal, Joana A., Author
Morkel, Markus1, Author              
Bramsen, Jesper B., Author
Herrmann, Bernhard G.1, Author              
Wittler, Lars2, Author              
Affiliations:
1Dept. of Developmental Genetics (Head: Bernhard G. Herrmann), Max Planck Institute for Molecular Genetics, Max Planck Society, Ihnestr. 73, 14195 Berlin, Germany, ou_1433548              
2Transgene Unit (Head: Lars Wittler), Scientific Service (Head: Manuela B. Urban), Max Planck Institute for Molecular Genetics, Max Planck Society, Ihnestr. 73, 14195 Berlin, Germany, ou_1479663              

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Free keywords: Brachyury; RNAi knockdown; Hypomorph; Notochord; Murine development; Axial development; Urorectal malformations; Mesoderm survival; Caudal regression
 Abstract: The T-box transcription factor BRACHYURY (T) is a key regulator of mesoderm formation during early development. Complete loss of T has been shown to lead to embryonic lethality around E10.0. Here we characterize an inducible miRNA-based in vivo knockdown mouse model of T, termed KD3-T, which exhibits a hypomorphic phenotype. KD3-T embryos display axial skeletal defects caused by apoptosis of paraxial mesoderm, which is accompanied by urorectal malformations resembling the murine uro-recto-caudal syndrome and human caudal regression syndrome phenotypes. We show that there is a reduction of T in the notochord of KD3-T embryos which results in impaired notochord differentiation and its subsequent loss, whereas levels of T in the tailbud are sufficient for axis extension and patterning. Furthermore, the notochord in KD3-T embryos adopts a neural character and loses its ability to act as a signaling center. Since KD3-T animals survive until birth, they are useful for examining later roles for T in the development of urorectal tissues.

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 Dates: 2012-09-072012-09-182012-12-01
 Publication Status: Published in print
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 Identifiers: DOI: 10.1016/j.ydbio.2012.09.003
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Title: Developmental Biology
Source Genre: Journal
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Publ. Info: San Diego [etc.] : Academic Press
Pages: - Volume / Issue: 372 (1) Sequence Number: - Start / End Page: 55 - 67 Identifier: ISSN: 0012-1606
CoNE: /journals/resource/954927680586