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  rsEGFP2 enables fast RESOLFT nanoscopy of living cells.

Grotjohann, T., Testa, I., Reuss, M., Brakemann, T., Eggeling, C., Hell, S. W., et al. (2012). rsEGFP2 enables fast RESOLFT nanoscopy of living cells. eLife, 1: e00248. doi:10.7554/eLife.00248.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-000E-B7F9-E Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0027-CDBA-B
Genre: Journal Article

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1676138.pdf (Publisher version), 4MB
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 Creators:
Grotjohann, T.1, Author              
Testa, I.1, Author              
Reuss, M.1, Author              
Brakemann, T.2, Author              
Eggeling, C.1, Author              
Hell, S. W.1, Author              
Jakobs, S.2, Author              
Affiliations:
1Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society, ou_578627              
2Research Group of Mitochondrial Structure and Dynamics, MPI for biophysical chemistry, Max Planck Society, ou_578566              

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 Abstract: The super-resolution microscopy called RESOLFT relying on fluorophore switching between longlived states, stands out by its coordinate-targeted sequential sample interrogation using low light levels. While RESOLFT has been shown to discern nanostructures in living cells, the reversibly photoswitchable green fluorescent protein (rsEGFP) employed in these experiments was switched rather slowly and recording lasted tens of minutes. We now report on the generation of rsEGFP2 providing faster switching and the use of this protein to demonstrate 25–250 times faster recordings.

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Language(s): eng - English
 Dates: 2012-12-31
 Publication Status: Published online
 Pages: 14
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 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.7554/eLife.00248
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Title: eLife
Source Genre: Journal
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Publ. Info: Cambridge : eLife Sciences Publications
Pages: - Volume / Issue: 1 Sequence Number: e00248 Start / End Page: - Identifier: Other: 2050-084X
CoNE: /journals/resource/2050-084X