English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Immunity to Intracellular Salmonella Depends on Surface-associated Antigens

Barat, S., Willer, Y., Rizos, K., Claudi, B., Mazé, A., Schemmer, A. K., et al. (2012). Immunity to Intracellular Salmonella Depends on Surface-associated Antigens. PLoS Pathogens, 8(10): e1002966. doi:10.1371/journal.ppat.1002966.

Item is

Basic

show hide
Genre: Journal Article
Alternative Title : PLoS Pathog.

Files

show Files
hide Files
:
PLoS_Pathogens_2012_8_e1002966.pdf (Publisher version), 864KB
Name:
PLoS_Pathogens_2012_8_e1002966.pdf
Description:
-
OA-Status:
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
Barat et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
License:
-

Locators

show

Creators

show
hide
 Creators:
Barat, Somedutta, Author
Willer, Yvonne, Author
Rizos, Konstantin1, Author           
Claudi, Beatrice, Author
Mazé, Alain, Author
Schemmer, Anne K., Author
Kirchhoff, Dennis, Author
Schmidt, Alexander, Author
Burton, Neil, Author
Bumann, Dirk1, Author           
Affiliations:
1Department of Molecular Biology, Max Planck Institute for Infection Biology, Max Planck Society, ou_1664147              

Content

show
hide
Free keywords: -
 Abstract: Invasive Salmonella infection is an important health problem that is worsening because of rising antimicrobial resistance and changing Salmonella serovar spectrum. Novel vaccines with broad serovar coverage are needed, but suitable protective antigens remain largely unknown. Here, we tested 37 broadly conserved Salmonella antigens in a mouse typhoid fever model, and identified antigen candidates that conferred partial protection against lethal disease. Antigen properties such as high in vivo abundance or immunodominance in convalescent individuals were not required for protectivity, but all promising antigen candidates were associated with the Salmonella surface. Surprisingly, this was not due to superior immunogenicity of surface antigens compared to internal antigens as had been suggested by previous studies and novel findings for CD4 T cell responses to model antigens. Confocal microscopy of infected tissues revealed that many live Salmonella resided alone in infected host macrophages with no damaged Salmonella releasing internal antigens in their vicinity. In the absence of accessible internal antigens, detection of these infected cells might require CD4 T cell recognition of Salmonella surface-associated antigens that could be processed and presented even from intact Salmonella. In conclusion, our findings might pave the way for development of an efficacious Salmonella vaccine with broad serovar coverage, and suggest a similar crucial role of surface antigens for immunity to both extracellular and intracellular pathogens.

Details

show
hide
Language(s): eng - English
 Dates: 2012-10
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: eDoc: 629453
ISI: 000310530300029
DOI: 10.1371/journal.ppat.1002966
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: PLoS Pathogens
  Alternative Title : PLoS Pathog.
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: SAN FRANCISCO : PUBLIC LIBRARY SCIENCE
Pages: - Volume / Issue: 8 (10) Sequence Number: e1002966 Start / End Page: - Identifier: ISSN: 1553-7374