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  Comparative Genomics and Transcriptomics of Propionibacterium acnes

Brzuszkiewicz, E., Weiner, J., Wollherr, A., Thürmer, A., Hüpeden, J., Lomholt, H. B., et al. (2011). Comparative Genomics and Transcriptomics of Propionibacterium acnes. PLoS ONE, 6(6): e21581. doi:10.1371/journal.pone.0021581.

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Genre: Journal Article
Alternative Title : PLoS One

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PLoS_One_2011_6_e21581.pdf (Publisher version), 3MB
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© 2011 Brzuszkiewicz et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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Brzuszkiewicz, Elzbieta, Author
Weiner, January1, Author           
Wollherr, Antje, Author
Thürmer, Andrea, Author
Hüpeden, Jennifer, Author
Lomholt, Hans B., Author
Kilian, Mogens, Author
Gottschalk, Gerhard, Author
Daniel, Rolf, Author
Mollenkopf, Hans-Joachim2, Author           
Meyer, Thomas F.3, Author           
Brüggemann, Holger3, Author           
Affiliations:
1Department of Immunology, Max Planck Institute for Infection Biology, Max Planck Society, ou_1664146              
2Core Facilities / Microarray, Max Planck Institute for Infection Biology, Max Planck Society, ou_1664141              
3Department of Molecular Biology, Max Planck Institute for Infection Biology, Max Planck Society, ou_1664147              

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 Abstract: The anaerobic Gram-positive bacterium Propionibacterium acnes is a human skin commensal that is occasionally associated with inflammatory diseases. Recent work has indicated that evolutionary distinct lineages of P. acnes play etiologic roles in disease while others are associated with maintenance of skin homeostasis. To shed light on the molecular basis for differential strain properties, we carried out genomic and transcriptomic analysis of distinct P. acnes strains. We sequenced the genome of the P. acnes strain 266, a type I-1a strain. Comparative genome analysis of strain 266 and four other P. acnes strains revealed that overall genome plasticity is relatively low; however, a number of island-like genomic regions, encoding a variety of putative virulence-associated and fitness traits differ between phylotypes, as judged from PCR analysis of a collection of P. acnes strains. Comparative transcriptome analysis of strains KPA171202 (type I-2) and 266 during exponential growth revealed inter-strain differences in gene expression of transport systems and metabolic pathways. In addition, transcript levels of genes encoding possible virulence factors such as dermatan-sulphate adhesin, polyunsaturated fatty acid isomerase, iron acquisition protein HtaA and lipase GehA were upregulated in strain 266. We investigated differential gene expression during exponential and stationary growth phases. Genes encoding components of the energy-conserving respiratory chain as well as secreted and virulence-associated factors were transcribed during the exponential phase, while the stationary growth phase was characterized by upregulation of genes involved in stress responses and amino acid metabolism. Our data highlight the genomic basis for strain diversity and identify, for the first time, the actively transcribed part of the genome, underlining the important role growth status plays in the inflammation-inducing activity of P. acnes. We argue that the disease-causing potential of different P. acnes strains is not only determined by the phylotype-specific genome content but also by variable gene expression.

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Language(s): eng - English
 Dates: 2011-06-27
 Publication Status: Issued
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 Rev. Type: Peer
 Identifiers: eDoc: 572750
ISI: 000292092600055
DOI: 10.1371/journal.pone.0021581
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Title: PLoS ONE
  Alternative Title : PLoS One
Source Genre: Journal
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Publ. Info: SAN FRANCISCO : PUBLIC LIBRARY SCIENCE
Pages: - Volume / Issue: 6 (6) Sequence Number: e21581 Start / End Page: - Identifier: ISSN: 1932-6203