English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Neutrophil elastase and myeloperoxidase regulate the formation of neutrophil extracellular traps

Papayannopoulos, V., Metzler, K. D., Hakkim, A., & Zychlinsky, A. (2010). Neutrophil elastase and myeloperoxidase regulate the formation of neutrophil extracellular traps. Journal of Cell Biology, 191(3), 677-691.

Item is

Basic

show hide
Genre: Journal Article
Alternative Title : J. Cell Biol.

Files

show Files

Locators

show

Creators

show
hide
 Creators:
Papayannopoulos, Venizelos1, Author              
Metzler, Kathleen D.1, Author              
Hakkim, Abdul2, Author
Zychlinsky, Arturo1, Author              
Affiliations:
1Department of Cellular Microbiology, Max Planck Institute for Infection Biology, Max Planck Society, ou_1664145              
2Max Planck Society, ou_persistent13              

Content

show
hide
Free keywords: -
 Abstract: Neutrophils release decondensed chromatin termed neutrophil extracellular traps (NETs) to trap and kill pathogens extracellularly Reactive oxygen species are required to initiate NET formation but the downstream molecular mechanism is unknown We show that upon activation, neutrophil elastase (NE) escapes from azurophilic granules and translocates to the nucleus, where it partially degrades specific histones, promoting chromatin decondensation Subsequently, myeloperoxidase synergizes with NE in driving chromatin decondensation independent of its enzymatic activity Accordingly, NE knockout mice do not form NETs in a pulmonary model of Klebsiella pneumoniae infection, which suggests that this defect may contribute to the immune deficiency of these mice This mechanism provides for a novel function for serine proteases and highly charged granular proteins in the regulation of chromatin density, and reveals that the oxidative burst induces a selective release of granular proteins into the cytoplasm through an unknown mechanism

Details

show
hide
Language(s): eng - English
 Dates: 2010-11-01
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: eDoc: 528367
ISI: 000284135700021
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Journal of Cell Biology
  Alternative Title : J. Cell Biol.
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 191 (3) Sequence Number: - Start / End Page: 677 - 691 Identifier: ISSN: 0021-9525