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  Experimental discovery of small RNAs in Staphylococcus aureus reveals a riboregulator of central metabolism

Bohn, C., Rigoulay, C., Chabelskaya, S., Sharma, C. M., Marchais, A., Skorski, P., et al. (2010). Experimental discovery of small RNAs in Staphylococcus aureus reveals a riboregulator of central metabolism. Nucleic Acids Research, 38(19), 6620-6636.

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Genre: Journal Article
Alternative Title : Nucleic Acids Res.

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Nucl_Acids_Res_2010_38_6620.pdf (Publisher version), 932KB
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© The Author(s) 2010. Published by Oxford University Press. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
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 Creators:
Bohn, Chantal, Author
Rigoulay, Candice, Author
Chabelskaya, Svetlana, Author
Sharma, Cynthia Mira1, Author              
Marchais, Antonin, Author
Skorski, Patricia, Author
Borezée-Durant, Elise, Author
Barbet, Romain, Author
Jacquet, Eric, Author
Jacq, Annick, Author
Gautheret, Daniel, Author
Felden, Brice, Author
Vogel, Jörg1, Author              
Bouloc, Philippe, Author
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1Max-Planck Research Group RNA Biology, Max Planck Institute for Infection Biology, Max Planck Society, ou_1664150              

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 Abstract: Using an experimental approach, we investigated the RNome of the pathogen Staphylococcus aureus to identify 30 small RNAs (sRNAs) including 14 that are newly confirmed. Among the latter, 10 are encoded in intergenic regions, three are generated by premature transcription termination associated with riboswitch activities, and one is expressed from the complementary strand of a transposase gene. The expression of four sRNAs increases during the transition from exponential to stationary phase. We focused our study on RsaE, an sRNA that is highly conserved in the bacillales order and is deleterious when over-expressed. We show that RsaE interacts in vitro with the 5′ region of opp3A mRNA, encoding an ABC transporter component, to prevent formation of the ribosomal initiation complex. A previous report showed that RsaE targets opp3B which is co-transcribed with opp3A. Thus, our results identify an unusual case of riboregulation where the same sRNA controls an operon mRNA by targeting two of its cistrons. A combination of biocomputational and transcriptional analyses revealed a remarkably coordinated RsaE-dependent downregulation of numerous metabolic enzymes involved in the citrate cycle and the folate-dependent one-carbon metabolism. As we observed that RsaE accumulates transiently in late exponential growth, we propose that RsaE functions to ensure a coordinate downregulation of the central metabolism when carbon sources become scarce.

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Language(s): eng - English
 Dates: 2010-10
 Publication Status: Published in print
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 Rev. Type: Peer
 Identifiers: eDoc: 529326
ISI: 000283682100034
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Title: Nucleic Acids Research
  Alternative Title : Nucleic Acids Res.
Source Genre: Journal
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Pages: - Volume / Issue: 38 (19) Sequence Number: - Start / End Page: 6620 - 6636 Identifier: ISSN: 0305-1048