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  Tarp regulates early Chlamydia-induced host cell survival through interactions with the human adaptor protein SHC1

Mehlitz, A., Banhart, S., Mäurer, A. P., Kaushansky, A., Gordus, A. G., Zielecki, J., et al. (2010). Tarp regulates early Chlamydia-induced host cell survival through interactions with the human adaptor protein SHC1. Journal of Cell Biology, 190(1), 143-157.

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Genre: Journal Article
Alternative Title : J. Cell Biol.

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© 2010 Mehlitz et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).
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 Creators:
Mehlitz, Adrian1, Author              
Banhart, Sebastian1, Author              
Mäurer, André P.2, Author
Kaushansky, Alexis, Author
Gordus, Andrew G., Author
Zielecki, Julia1, Author              
MacBeath, Gavin, Author
Meyer, Thomas F.1, Author              
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1Department of Molecular Biology, Max Planck Institute for Infection Biology, Max Planck Society, ou_1664147              
2Max Planck Society, ou_persistent13              

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 Abstract: Many bacterial pathogens translocate effector proteins into host cells to manipulate host cell functions. Here, we used a protein microarray comprising virtually all human SRC homology 2 (SH2) and phosphotyrosine binding domains to comprehensively and quantitatively assess interactions between host cell proteins and the early phase Chlamydia trachomatis effector protein translocated actin-recruiting phosphoprotein (Tarp), which is rapidly tyrosine phosphorylated upon host cell entry. We discovered numerous novel interactions between human SH2 domains and phosphopeptides derived from Tarp. The adaptor protein SHC1 was among Tarp's strongest interaction partners. Transcriptome analysis of SHC1-dependent gene regulation during infection indicated that SHC1 regulates apoptosis- and growth-related genes. SHC1 knockdown sensitized infected host cells to tumor necrosis factor-induced apoptosis. Collectively, our findings reveal a critical role for SHC1 in early C. trachomatis-induced cell survival and suggest that Tarp functions as a multivalent phosphorylation-dependent signaling hub that is important during the early phase of chlamydial infection.

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Language(s): eng - English
 Dates: 2010-07-12
 Publication Status: Published in print
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 Rev. Type: Peer
 Identifiers: eDoc: 532541
ISI: 000279792700016
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Title: Journal of Cell Biology
  Alternative Title : J. Cell Biol.
Source Genre: Journal
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Pages: - Volume / Issue: 190 (1) Sequence Number: - Start / End Page: 143 - 157 Identifier: ISSN: 0021-9525