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  Identification of T-cell antigens specific for latent mycobacterium tuberculosis infection.

Schuck, S. D., Mueller, H., Kunitz, F., Neher, A., Hoffmann, H., Franken, K. L. C. M., et al. (2009). Identification of T-cell antigens specific for latent mycobacterium tuberculosis infection. PLoS ONE, 4(5): e5590.

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PLoS_ONE_2009_4_e5590.pdf (Publisher version), 896KB
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© 2009 Schuck et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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Schuck, Sebastian D.1, Author              
Mueller, Henrik1, Author              
Kunitz, Frank, Author
Neher, Albert, Author
Hoffmann, Harald, Author
Franken, Kees L. C. M., Author
Repsilber, Dirk, Author
Ottenhoff, Tom H. M., Author
Kaufmann, Stefan H. E.1, Author              
Jacobsen, Marc1, Author              
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1Department of Immunology, Max Planck Institute for Infection Biology, Max Planck Society, ou_1664146              

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 Abstract: BACKGROUND: T-cell responses against dormancy-, resuscitation-, and reactivation-associated antigens of Mycobacterium tuberculosis are candidate biomarkers of latent infection in humans. METHODOLOGY/PRINCIPAL FINDINGS: We established an assay based on two rounds of in vitro restimulation and intracellular cytokine analysis that detects T-cell responses to antigens expressed during latent M. tuberculosis infection. Comparison between active pulmonary tuberculosis (TB) patients and healthy latently M. tuberculosis-infected donors (LTBI) revealed significantly higher T-cell responses against 7 of 35 tested M. tuberculosis latency-associated antigens in LTBI. Notably, T cells specific for Rv3407 were exclusively detected in LTBI but not in TB patients. The T-cell IFNgamma response against Rv3407 in individual donors was the most influential factor in discrimination analysis that classified TB patients and LTBI with 83% accuracy using cross-validation. Rv3407 peptide pool stimulations revealed distinct candidate epitopes in four LTBI. CONCLUSIONS: Our findings further support the hypothesis that the latency-associated antigens can be exploited as biomarkers for LTBI.

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Language(s): eng - English
 Dates: 2009-05
 Publication Status: Published in print
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 Identifiers: eDoc: 444325
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Title: PLoS ONE
Source Genre: Journal
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Pages: - Volume / Issue: 4 (5) Sequence Number: e5590 Start / End Page: - Identifier: ISSN: 1932-6203