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  The speciation of the proteome

Jungblut, P. R., Holzhütter, H.-G., Apweiler, R., & Schlüter, H. (2008). The speciation of the proteome. Chemistry Central Journal, 2: 16.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-000E-C1A0-3 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-000E-C1A1-1
Genre: Journal Article
Alternative Title : Chem. Cent. J.

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Chem_centr_j_2008_2_16.pdf (Publisher version), 296KB
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© 2008 Jungblut et al This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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Jungblut, Peter R.1, Author              
Holzhütter, Hermann-Georg, Author
Apweiler, Rolf, Author
Schlüter, Hartmut, Author
Affiliations:
1Core Facilities / Proteinanalysis, Max Planck Institute for Infection Biology, Max Planck Society, ou_1664143              

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 Abstract: Abstract Introduction In proteomics a paradox situation developed in the last years. At one side it is basic knowledge that proteins are post-translationally modified and occur in different isoforms. At the other side the protein expression concept disclaims post-translational modifications by connecting protein names directly with function. Discussion Optimal proteome coverage is today reached by bottom-up liquid chromatography/mass spectrometry. But quantification at the peptide level in shotgun or bottom-up approaches by liquid chromatography and mass spectrometry is completely ignoring that a special peptide may exist in an unmodified form and in several-fold modified forms. The acceptance of the protein species concept is a basic prerequisite for meaningful quantitative analyses in functional proteomics. In discovery approaches only top-down analyses, separating the protein species before digestion, identification and quantification by two-dimensional gel electrophoresis or protein liquid chromatography, allow the correlation between changes of a biological situation and function. Conclusion To obtain biological relevant information kinetics and systems biology have to be performed at the protein species level, which is the major challenge in proteomics today.

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Language(s): eng - English
 Dates: 2008-07-18
 Publication Status: Published in print
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 Rev. Type: Peer
 Identifiers: eDoc: 399860
ISI: 000259067800001
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Title: Chemistry Central Journal
  Alternative Title : Chem. Cent. J.
Source Genre: Journal
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Pages: - Volume / Issue: 2 Sequence Number: 16 Start / End Page: - Identifier: ISSN: 1752-153X