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  Immunoproteomics

Krah, A., & Jungblut, P. R. (2003). Immunoproteomics. In J. Decker (Ed.), Molecular diagnosis of infectious diseases (pp. 19-32). Totowa, N.J.: Humana.

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Mol_Diagn_Infect_Dis_2003_19.pdf (Publisher version), 635KB
 
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 Creators:
Krah, Alexander1, Author              
Jungblut, Peter R.2, Author              
Affiliations:
1Department of Molecular Biology, Max Planck Institute for Infection Biology, Max Planck Society, ou_1664147              
2Core Facilities / Proteinanalysis, Max Planck Institute for Infection Biology, Max Planck Society, ou_1664143              

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Free keywords: Two-dimensional electrophoresis; immunoproteome; diagnostics; therapy; vaccination; mass spectrometry; peptide mass fingerprinting; proteome database
 Abstract: Two-dimensional electrophoresis results in an adequate resolution of the proteome of microorganisms to allow the detection and identification of specific antigens after blotting on membranes and overlaying the protein pattern with patient's sera. The complement of all identified antigens presents the immunoproteome of a microorganism. All the antigens specific for a microorganism or even for a disease are identified by mass spectrometry. For identification, peptide mass fingerprinting is used, and post-translational modifications are detected by mass spectrometry MS/MS techniques. High-resolution two-dimensional electrophoresis and unambiguous identification are prerequisites for reliable results. After statistical analysis, the resulting antigens are candidates for diagnosis or vaccination and targets for therapy.

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Language(s): eng - English
 Dates: 2003-12
 Publication Status: Published in print
 Pages: -
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 Rev. Type: -
 Identifiers: eDoc: 224290
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Title: Molecular diagnosis of infectious diseases
Source Genre: Book
 Creator(s):
Decker, Jochen, Editor
Affiliations:
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Publ. Info: Totowa, N.J. : Humana
Pages: - Volume / Issue: - Sequence Number: - Start / End Page: 19 - 32 Identifier: ISBN: 1-588-29221-5

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Title: Methods in molecular medicine
Source Genre: Series
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Pages: - Volume / Issue: 94 Sequence Number: - Start / End Page: - Identifier: -