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  Sample preparation and current applications of liquid chromatography for the determination of non-structural carbohydrates in plants

Raessler, M. (2011). Sample preparation and current applications of liquid chromatography for the determination of non-structural carbohydrates in plants. TRAC - Trends in Analytical Chemistry, 30(11), 1833-1843. doi:10.1016/j.trac.2011.06.013.

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資料種別: 学術論文

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BGC1549.pdf (出版社版), 417KB
 
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 作成者:
Raessler, M.1, 著者           
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1Service Facility Spectrometry, Dr. M. Raessler, Max Planck Institute for Biogeochemistry, Max Planck Society, ou_1497771              

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キーワード: Evaporative light scattering (ELS) Free carbohydrate (FC) Liquid chromatography (LC) Mass spectrometry (MS) Non-structural carbohydrate (NSC) Plant sample Polysaccharide Pulsed amperometry (PA) Refractive index (RI) Sample preparation anion-exchange chromatography pulsed amperometric detection ionization mass-spectrometry light-scattering detection scots pine-seedlings allium-cepa l sugar phosphates carbon extraction tissues
 要旨: This article summarizes the current methods of determination of non-structural carbohydrates (NSCs) in plant samples based on liquid chromatography (LC). NSCs comprise several types of carbohydrates: sugar alcohols (e.g., sorbitol), monosaccharides (e.g., glucose and fructose), disaccharides (e.g., sucrose), oligosaccharides (e.g., raffinose) and polysaccharides [e.g., starch and polyfructans (e.g., inulin)]. NSCs are important in plant metabolism and have to be strictly distinguished from all sorts of structural carbohydrates (e.g., polysaccharide cellulose) that make up the backbone of the plants. Consequently, preservation of structural carbohydrates is a crucial step during sample preparation for NSC determination and is therefore addressed. Sugar alcohols, monosaccharides, disaccharides and those oligosaccharides that are easily soluble in polar solvents can be analyzed directly by high-performance LC. They are also referred to as free carbohydrates (FCs). However, polysaccharides are generally submitted to hydrolyzation into monomers prior to their quantitative analysis. This can be done either chemically, using acids, or enzymatically - both methods are discussed. For identification and quantification of the NSCs after LC separation, the following detectors are used: pulsed amperometry, refractive index, evaporate light scattering and finally, mass spectrometry. (C) 2011 Elsevier Ltd. All rights reserved.

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言語: eng - English
 日付: 2011
 出版の状態: 出版
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 識別子(DOI, ISBNなど): DOI: 10.1016/j.trac.2011.06.013
ISI: ://WOS:000298621500018
その他: BGC1549
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出版物 1

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出版物名: TRAC - Trends in Analytical Chemistry
種別: 学術雑誌
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出版社, 出版地: -
ページ: - 巻号: 30 (11) 通巻号: - 開始・終了ページ: 1833 - 1843 識別子(ISBN, ISSN, DOIなど): ISSN: 0165-9936