English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
 
 
DownloadE-Mail
  Sample preparation and current applications of liquid chromatography for the determination of non-structural carbohydrates in plants

Raessler, M. (2011). Sample preparation and current applications of liquid chromatography for the determination of non-structural carbohydrates in plants. TRAC - Trends in Analytical Chemistry, 30(11), 1833-1843. doi:10.1016/j.trac.2011.06.013.

Item is

Files

show Files
hide Files
:
BGC1549.pdf (Publisher version), 417KB
 
File Permalink:
-
Name:
BGC1549.pdf
Description:
-
OA-Status:
Visibility:
Restricted (Max Planck Institute for Biogeochemistry, MJBK; )
MIME-Type / Checksum:
application/octet-stream
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show

Creators

show
hide
 Creators:
Raessler, M.1, Author           
Affiliations:
1Service Facility Spectrometry, Dr. M. Raessler, Max Planck Institute for Biogeochemistry, Max Planck Society, ou_1497771              

Content

show
hide
Free keywords: Evaporative light scattering (ELS) Free carbohydrate (FC) Liquid chromatography (LC) Mass spectrometry (MS) Non-structural carbohydrate (NSC) Plant sample Polysaccharide Pulsed amperometry (PA) Refractive index (RI) Sample preparation anion-exchange chromatography pulsed amperometric detection ionization mass-spectrometry light-scattering detection scots pine-seedlings allium-cepa l sugar phosphates carbon extraction tissues
 Abstract: This article summarizes the current methods of determination of non-structural carbohydrates (NSCs) in plant samples based on liquid chromatography (LC). NSCs comprise several types of carbohydrates: sugar alcohols (e.g., sorbitol), monosaccharides (e.g., glucose and fructose), disaccharides (e.g., sucrose), oligosaccharides (e.g., raffinose) and polysaccharides [e.g., starch and polyfructans (e.g., inulin)]. NSCs are important in plant metabolism and have to be strictly distinguished from all sorts of structural carbohydrates (e.g., polysaccharide cellulose) that make up the backbone of the plants. Consequently, preservation of structural carbohydrates is a crucial step during sample preparation for NSC determination and is therefore addressed. Sugar alcohols, monosaccharides, disaccharides and those oligosaccharides that are easily soluble in polar solvents can be analyzed directly by high-performance LC. They are also referred to as free carbohydrates (FCs). However, polysaccharides are generally submitted to hydrolyzation into monomers prior to their quantitative analysis. This can be done either chemically, using acids, or enzymatically - both methods are discussed. For identification and quantification of the NSCs after LC separation, the following detectors are used: pulsed amperometry, refractive index, evaporate light scattering and finally, mass spectrometry. (C) 2011 Elsevier Ltd. All rights reserved.

Details

show
hide
Language(s): eng - English
 Dates: 2011
 Publication Status: Issued
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: DOI: 10.1016/j.trac.2011.06.013
ISI: ://WOS:000298621500018
Other: BGC1549
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: TRAC - Trends in Analytical Chemistry
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 30 (11) Sequence Number: - Start / End Page: 1833 - 1843 Identifier: ISSN: 0165-9936