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  The essence on mass spectrometry based microbial diagnostics

Kliem, M., & Sauer, S. (2012). The essence on mass spectrometry based microbial diagnostics. Current Opinion in Microbiology, 15(3), 397-402. doi:10.1016/j.mib.2012.02.006.

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CurrOpMicrobiolReview.pdf (Publisher version), 312KB
 
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In recent years, matrix-assisted laser desorption/ionisation time-of-flight (MALDI-TOF) mass spectrometry has become an important bioanalytical method to detect profiles of proteins and peptides derived from whole bacterial cells. This accurate molecular-phenotypic method can be easily applied to robustly detect bacteria on the genus, species and in some cases on the subspecies level. Standardised laboratory protocols for the preparation of abundant bacterial proteins and the development of tailored data analysis software, as well as high-quality databases of microbial reference mass spectra, made the procedure attractive to replace phenotypic or biochemical procedures for identification of bacteria and other microorganisms. Moreover, genotypic and functional mass spectrometry based methods to detect for example bacterial strains or antibiotic resistance may become useful in the coming years. In general, mass spectrometry is a powerful tool to facilitate routine microbial diagnostics.
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© 2012 Elsevier Ltd
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 Creators:
Kliem, Magdalena1, Author              
Sauer, Sascha1, Author              
Affiliations:
1Nutrigenomics and Gene Regulation (Sascha Sauer), Independent Junior Research Groups (OWL), Max Planck Institute for Molecular Genetics, Max Planck Society, Ihnestr. 63-73, 14195 Berlin, ou_1479662              

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 Abstract: In recent years, matrix-assisted laser desorption/ionisation time-of-flight (MALDI-TOF) mass spectrometry has become an important bioanalytical method to detect profiles of proteins and peptides derived from whole bacterial cells. This accurate molecular-phenotypic method can be easily applied to robustly detect bacteria on the genus, species and in some cases on the subspecies level. Standardised laboratory protocols for the preparation of abundant bacterial proteins and the development of tailored data analysis software, as well as high-quality databases of microbial reference mass spectra, made the procedure attractive to replace phenotypic or biochemical procedures for identification of bacteria and other microorganisms. Moreover, genotypic and functional mass spectrometry based methods to detect for example bacterial strains or antibiotic resistance may become useful in the coming years. In general, mass spectrometry is a powerful tool to facilitate routine microbial diagnostics.

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Language(s): eng - English
 Dates: 2012-06
 Publication Status: Published in print
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 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1016/j.mib.2012.02.006
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Title: Current Opinion in Microbiology
Source Genre: Journal
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Publ. Info: London : Elsevier Current Trends
Pages: - Volume / Issue: 15 (3) Sequence Number: - Start / End Page: 397 - 402 Identifier: ISSN: 1369-5274
CoNE: https://pure.mpg.de/cone/journals/resource/954925620175