English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Compounds associated with algal surfaces mediate epiphytic colonization of the marine macroalga Fucus vesiculosus

Lachnit, T., Fischer, M., Künzel, S., Baines, J. F., & Harder, T. (2013). Compounds associated with algal surfaces mediate epiphytic colonization of the marine macroalga Fucus vesiculosus. FEMS Microbiology Ecology, 84(2), 411-420. doi:10.1111/1574-6941.12071.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-000E-E55B-7 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-000E-E55D-3
Genre: Journal Article

Files

show Files
hide Files
:
fem12071.pdf (Publisher version), 410KB
 
File Permalink:
-
Name:
fem12071.pdf
Description:
-
Visibility:
Restricted (Max Planck Institute for Evolutionary Biology, Plön; )
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show

Creators

show
hide
 Creators:
Lachnit, Tim, Author
Fischer, Matthias, Author
Künzel, Sven1, Author              
Baines, John F.2, Author              
Harder, Tilmann, Author
Affiliations:
1Department Evolutionary Genetics, Max Planck Institute for Evolutionary Biology, Max Planck Society, ou_1445635              
2Guest Group Evolutionary Genomics, Max Planck Institute for Evolutionary Biology, Max Planck Society, ou_1445638              

Content

show
hide
Free keywords: macroalga; bacterial community; surface chemistry; antifouling; settlement control; seaweed
 Abstract: The macroalga Fucus vesiculosus carries a specific community of surface bacteria. To identify chemical compounds that possibly mediate abundance and community composition of algae-associated bacteria, we tested the effect of surface-available algal compounds on bacterial settlement and community composition under field conditions. Compounds on algal thalli were separated from the surface by extraction with organic solvents and investigated on growth inhibition and settlement of bacterial isolates. Based on in vitro data, partially purified extract fractions were then exposed to bacterial colonizers in vivo followed by bacterial enumeration and community analysis. The polar fraction of the algal surface extract revealed a significant profouling effect for Vibrionales, whereas the nonpolar fraction – containing the xanthophyll pigment fucoxanthin and other unidentified nonpolar surface compounds – revealed a significant 80% reduction of surface colonizing bacteria. The analysis of bacterial surface communities by 454 pyrosequencing demonstrated that the antifouling activity of nonpolar algal surface compounds was targeting the abundance of natural bacterial colonizers rather than the relative composition of bacterial members within the community. Moreover, the bacterial community composition on F. vesiculosus was markedly different from artificial control substrates and chemically manipulated experimental treatments, suggesting that other, nonextractable surface features and/or physical properties render algal-specific epiphytic bacterial communities.

Details

show
hide
Language(s): eng - English
 Dates: 2013-01-082012-06-222013-01-082013-02-052013-05
 Publication Status: Published in print
 Pages: 10 S.
 Publishing info: -
 Table of Contents: -
 Rev. Method: -
 Identifiers: DOI: 10.1111/1574-6941.12071
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: FEMS Microbiology Ecology
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Wiley-Blackwell - STM
Pages: - Volume / Issue: 84 (2) Sequence Number: - Start / End Page: 411 - 420 Identifier: ISSN: 0168-6496 (print)
ISSN: 1574-6941 (online)
CoNE: /journals/resource/954925526820_1