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  Characterization of herpes simplex virus type 1 L-particle assembly and egress in hippocampal neurones by electron cryo-tomography

Ibiricu, I., Maurer, U. E., & Grünewald, K. (2013). Characterization of herpes simplex virus type 1 L-particle assembly and egress in hippocampal neurones by electron cryo-tomography. CELLULAR MICROBIOLOGY, 15(2), 285-291. doi:10.1111/cmi.12093.

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 Creators:
Ibiricu, Iosune1, Author              
Maurer, Ulrike Elisabeth1, Author              
Grünewald, Kay2, Author              
Affiliations:
1Baumeister, Wolfgang / Molecular Structural Biology, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565142              
2Former Research Groups, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565145              

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Free keywords: CRYOELECTRON TOMOGRAPHY; COATED VESICLES; CLATHRIN; TRANSPORT; COPI; MICROSCOPY; INFECTION; TEGUMENT; REPLICATION; MATURATION
 Abstract: Visualizing virus-host interactions in situ inside infected cells by electron cryo-tomography provides unperturbed snapshots of the infection process. Here we focus on the assembly and egress pathway of herpesviruses. Cells infected with herpes simplex virus 1 produce and release not only infective virions but also non-infectious light particles (L-particles). L-particles are devoid of viral capsids and genomes. In this study, we analysed L-particle assembly and egress pathways in cultured dissociated hippocampus neurones by electron cryo-tomography. Virion and L-particle formation occurred in close proximity, suggesting shared assembly and exit pathways. Clathrin-like coats were occasionally associated with L-particle and virion assembly sites. Further, we compared the three-dimensional ultrastructure of intracellular and extracellular L-particles and quantified their diameters and the abundance of inclusion bodies contained.

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Language(s): eng - English
 Dates: 2013-02
 Publication Status: Published in print
 Pages: 7
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: ISI: 000314587000012
DOI: 10.1111/cmi.12093
 Degree: -

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Title: CELLULAR MICROBIOLOGY
Source Genre: Journal
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Publ. Info: 111 RIVER ST, HOBOKEN 07030-5774, NJ USA : WILEY-BLACKWELL
Pages: - Volume / Issue: 15 (2) Sequence Number: - Start / End Page: 285 - 291 Identifier: ISSN: 1462-5814