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  Multiple cis-regulatory elements are involved in the complex regulation of the sieve element-specific MtSEO-F1 promoter from Medicago truncatula

Bucsenez, M., Rüping, B., Behrens, S., Twyman, R. M., Noll, G. A., & Prüfer, D. (2012). Multiple cis-regulatory elements are involved in the complex regulation of the sieve element-specific MtSEO-F1 promoter from Medicago truncatula. Plant Biology, 14(5), 714-724. doi:10.1111/j.1438-8677.2011.00556.x.

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 Creators:
Bucsenez, M.1, Author
Rüping, B.2, Author
Behrens, S.3, Author              
Twyman, R. M.4, Author
Noll, G. A.2, Author
Prüfer, D.1, Author
Affiliations:
1Fraunhofer Institute for Molecular Biology and Applied Ecology (IME), Aachen, Germany, ou_persistent13              
2Institut für Biologie und Biotechnologie der Pflanzen, Westfälische Wilhelms-Universität Münster, Münster, Germany, ou_persistent22              
3Dept. of Computational Molecular Biology (Head: Martin Vingron), Max Planck Institute for Molecular Genetics, Max Planck Society, Berlin, Germany, ou_1433547              
4Department of Biological Sciences, University of Warwick, Coventry, UK, ou_persistent22              

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Free keywords: Companion cell; forisome; immature sieve element; phloem; promoter; reporter gene; sieve element occlusion
 Abstract: The sieve element occlusion (SEO) gene family includes several members that are expressed specifically in immature sieve elements (SEs) in the developing phloem of dicotyledonous plants. To determine how this restricted expression profile is achieved, we analysed the SE-specific Medicago truncatula SEO-F1 promoter (PMtSEO-F1) by constructing deletion, substitution and hybrid constructs and testing them in transgenic tobacco plants using green fluorescent protein as a reporter. This revealed four promoter regions, each containing cis-regulatory elements that activate transcription in SEs. One of these segments also contained sufficient information to suppress PMtSEO-F1 transcription in the phloem companion cells (CCs). Subsequent in silico analysis revealed several candidate cis-regulatory elements that PMtSEO-F1 shares with other SEO promoters. These putative sieve element boxes (PSE boxes) are promising candidates for cis-regulatory elements controlling the SE-specific expression of PMtSEO-F1.

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Language(s): eng - English
 Dates: 2012-03-082012-09
 Publication Status: Published in print
 Pages: -
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 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1111/j.1438-8677.2011.00556.x
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Title: Plant Biology
Source Genre: Journal
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Publ. Info: Stuttgart : G. Thieme Verlag
Pages: - Volume / Issue: 14 (5) Sequence Number: - Start / End Page: 714 - 724 Identifier: ISSN: 1435-8603
CoNE: https://pure.mpg.de/cone/journals/resource/954925624284