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  Med12 is essential for early mouse development and for canonical Wnt and Wnt/PCP signaling.

Rocha, P. P., Scholze, M., Bleiß, W., & Schrewe, H. (2010). Med12 is essential for early mouse development and for canonical Wnt and Wnt/PCP signaling. Development, 137, 2723-2731. doi:10.1242/dev.053660.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-000E-E8AE-C Version Permalink: http://hdl.handle.net/11858/00-001M-0000-000E-E8AF-A
Genre: Journal Article

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© 2010. Published by The Company of Biologists Ltd
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 Creators:
Rocha, Pedro P.1, Author
Scholze, Manuela2, Author              
Bleiß, Wilfried3, Author
Schrewe, Heinrich2, Author              
Affiliations:
1 Institute of Medical Genetics, Charité-University Medicine Berlin, Berlin 12200, Germany, ou_persistent22              
2Dept. of Developmental Genetics (Head: Bernhard G. Herrmann), Max Planck Institute for Molecular Genetics, Max Planck Society, Ihnestr. 73, 14195 Berlin, Germany, ou_1433548              
3Department of Molecular Parasitology, Humboldt-University Berlin, 10115 Berlin, Germany, ou_persistent22              

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Free keywords: Gastrulation Mediator Neural tube closure Planar cell polarity Somitogenesis Wnt signaling Mouse
 Abstract: The Mediator complex is commonly seen as a molecular bridge that connects DNA-bound transcription factors to the RNA polymerase II (Pol II) machinery. It is a large complex of 30 subunits that is present in all eukaryotes. The Med12 subunit has been implicated not only in the regulation of Pol II activity, but also in the binding of transcription factors to the bulk of the Mediator complex. We targeted Med12 in mouse embryonic stem cells to investigate the in vivo function of this subunit. We report here the developmental defects of Med12 hypomorphic mutants that have a drastic reduction in Med12 protein levels. These mutants fail to develop beyond embryonic day 10 and have severe defects in neural tube closure, axis elongation, somitogenesis and heart formation. We show that in Med12 hypomorphic embryos, the Wnt/planar cell polarity pathway is disrupted and that canonical Wnt/β-catenin signaling is impaired. In agreement with this, embryos that are incapable of Med12 expression failed to establish the anterior visceral endoderm or activate brachyury expression, and did not complete gastrulation.

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Language(s): eng - English
 Dates: 2010-07-142010-08
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1242/dev.053660
 Degree: -

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Title: Development
  Other : Development
Source Genre: Journal
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Publ. Info: Cambridge, Cambridgeshire : Company of Biologists
Pages: - Volume / Issue: 137 Sequence Number: - Start / End Page: 2723 - 2731 Identifier: ISSN: 0950-1991
CoNE: /journals/resource/954927546241