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  Minimum Information Specification For In Situ Hybridization and Immunohistochemistry Experiments (MISFISHIE).

Deutsch, E. W., Ball, C. A., Berman, J. J., Bova, G. S., Brazma, A., Bumgarner, R. E., et al. (2008). Minimum Information Specification For In Situ Hybridization and Immunohistochemistry Experiments (MISFISHIE). Nature biotechnology, 26, 305-312. doi:10.1038/nbt1391.

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Deutsch.pdf (Publisher version), 249KB
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Deutsch, Eric W., Author
Ball, Catherine A., Author
Berman, Jules J., Author
Bova, G. Steven, Author
Brazma, Alvis, Author
Bumgarner, Roger E., Author
Campbell, David, Author
Causton, Helen C., Author
Christiansen, Jeffrey H., Author
Daian, Fabrice, Author
Dauga, Delphine, Author
Davidson, Duncan R., Author
Gimenez, Gregory, Author
Goo, Young Ah , Author
Grimmond, Sean, Author
Henrich, Thorsten , Author
Herrmann, Bernhard G.1, Author           
Johnson, Michael H., Author
Korb, Martin, Author
Mills, Jason C. , Author
Oudes, Asa J., AuthorParkinson, Helen E., AuthorPascal, Laura E., AuthorPollet, Nicolas, AuthorQuackenbush, John, AuthorRamialison, Mirana, AuthorRingwald, Martin, AuthorSalgado, David, AuthorSansone, Susanna-Assunta , AuthorSherlock, Gavin, AuthorStoeckert, Jr, Christian J. , AuthorSwedlow, Jason , AuthorTaylor, Ronald C. , AuthorWalashek, Laura, AuthorWarford, Anthony, AuthorWilkinson, David G. , AuthorZhou, Yi, AuthorZon, Leonard I., AuthorLiu, Alvin Y., AuthorTrue, Lawrence D. , Author more..
Affiliations:
1Dept. of Developmental Genetics (Head: Bernhard G. Herrmann), Max Planck Institute for Molecular Genetics, Max Planck Society, Ihnestr. 73, berlin, Germany, ou_1433548              

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 Abstract: One purpose of the biomedical literature is to report results in sufficient detail that the methods of data collection and analysis can be independently replicated and verified. Here we present reporting guidelines for gene expression localization experiments: the minimum information specification for in situ hybridization and immunohistochemistry experiments (MISFISHIE). MISFISHIE is modeled after the Minimum Information About a Microarray Experiment (MIAME) specification for microarray experiments. Both guidelines define what information should be reported without dictating a format for encoding that information. MISFISHIE describes six types of information to be provided for each experiment: experimental design, biomaterials and treatments, reporters, staining, imaging data and image characterizations. This specification has benefited the consortium within which it was developed and is expected to benefit the wider research community. We welcome feedback from the scientific community to help improve our proposal.

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Language(s): eng - English
 Dates: 2008-03-072008
 Publication Status: Issued
 Pages: -
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 Identifiers: DOI: 10.1038/nbt1391
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Title: Nature biotechnology
Source Genre: Journal
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Publ. Info: New York : Gale Group Inc.
Pages: - Volume / Issue: 26 Sequence Number: - Start / End Page: 305 - 312 Identifier: ISSN: 1087-0156
CoNE: https://pure.mpg.de/cone/journals/resource/954926980065