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  Comparative phenotypic analysis of the two major splice isoforms of phosphatidylinositol phosphate kinase type I gamma in vivo

Legate, K. R., Montag, D., Böttcher, R. T., Takahashi, S., & Fässler, R. (2012). Comparative phenotypic analysis of the two major splice isoforms of phosphatidylinositol phosphate kinase type I gamma in vivo. JOURNAL OF CELL SCIENCE, 125(23), 5636-5646. doi:10.1242/jcs.102145.

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Legate, Kyle R.1, Author              
Montag, Dirk2, Author
Böttcher, Ralph T.1, Author              
Takahashi, Seiichiro1, Author              
Fässler, Reinhard1, Author              
Affiliations:
1Fässler, Reinhard / Molecular Medicine, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565147              
2external, ou_persistent22              

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Free keywords: MOTOR COORDINATION; INTERSTITIAL-CELLS; PLASMA-MEMBRANE; FOCAL ADHESIONS; SMOOTH-MUSCLE; PIP KINASE; C-KIT; MICE; 5-KINASE; ASSOCIATIONPIP kinase; Clathrin-mediated endocytosis; Calcium signalling; Knockout mouse;
 Abstract: Localized production of polyphosphoinositides is critical for their signaling function. To examine the biological relevance of specific pools of phosphatidylinositol 4,5-bisphosphate we compared the consequences of genetically ablating all isoforms of phosphatidylinositol phosphate (PIP) kinase type I gamma (PIPKI gamma), encoded by the gene Pip5k1c, versus ablation of a specific splice isoform, PIPKI gamma_i2, with respect to three reported PIPKI gamma functions. Ablation of PIPKI gamma_i2 caused a neuron-specific endocytosis defect similar to that found in PIPKI gamma(-/-) mice, while agonist-induced calcium signaling was reduced in PIPKI gamma(-/-) cells, but was not affected in the absence of PIPKI gamma_i2. A reported contribution of PIPKI gamma to epithelial integrity was not evident in PIPKI gamma(-/-) mice. Given that mice lacking PIPKI gamma_i2 live a normal lifespan whereas PIPKI gamma(-/-) mice die shortly after birth, we propose that PIPKI gamma-mediated metabotropic calcium signaling may represent an essential function of PIPKI gamma, whereas functions specific to the PIPKI gamma_i2 splice isoform are not essential for survival.

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Language(s): eng - English
 Dates: 2012-12-01
 Publication Status: Published in print
 Pages: 11
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: ISI: 000315164200006
DOI: 10.1242/jcs.102145
 Degree: -

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Title: JOURNAL OF CELL SCIENCE
Source Genre: Journal
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Publ. Info: BIDDER BUILDING CAMBRIDGE COMMERCIAL PARK COWLEY RD, CAMBRIDGE CB4 4DL, CAMBS, ENGLAND : COMPANY OF BIOLOGISTS LTD
Pages: - Volume / Issue: 125 (23) Sequence Number: - Start / End Page: 5636 - 5646 Identifier: ISSN: 0021-9533