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  Maturation of mammalian H/ACA box snoRNAs: PAPD5-dependent adenylation and PARN-dependent trimming

Berndt, H., Harnisch, C., Rammelt, C., Stohr, N., Zirkel, A., Dohm, J. C., et al. (2012). Maturation of mammalian H/ACA box snoRNAs: PAPD5-dependent adenylation and PARN-dependent trimming. RNA-A Publication of the RNA Society, 18(5), 958-972. doi:10.1261/rna.032292.112.

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Berndt, H., Author
Harnisch, C., Author
Rammelt, C., Author
Stohr, N., Author
Zirkel, A., Author
Dohm, J. C.1, 2, Author              
Himmelbauer, H.1, 2, Author              
Tavanez, J. P., Author
Hüttelmaier, S., Author
Wahle, E., Author
Affiliations:
1Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society, Berlin, Germany, ou_1433550              
2Centre for Genomic Regulation (CRG) and UPF, Barcelona, Spain , ou_persistent22              

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Free keywords: Base Sequence Catalysis Cell Nucleolus/metabolism Coiled Bodies/metabolism Exoribonucleases/genetics/*metabolism Exosome Multienzyme Ribonuclease Complex Humans Nuclear Proteins/metabolism Nucleotide Motifs Polyadenylation Protein Transport RNA Editing RNA Nucleotidyltransferases/*metabolism RNA, Small Nucleolar/*chemistry/*metabolism
 Abstract: Small nucleolar and small Cajal body RNAs (snoRNAs and scaRNAs) of the H/ACA box and C/D box type are generated by exonucleolytic shortening of longer precursors. Removal of the last few nucleotides at the 3' end is known to be a distinct step. We report that, in human cells, knock-down of the poly(A) specific ribonuclease (PARN), previously implicated only in mRNA metabolism, causes the accumulation of oligoadenylated processing intermediates of H/ACA box but not C/D box RNAs. In agreement with a role of PARN in snoRNA and scaRNA processing, the enzyme is concentrated in nucleoli and Cajal bodies. Oligo(A) tails are attached to a short stub of intron sequence remaining beyond the mature 3' end of the snoRNAs. The noncanonical poly(A) polymerase PAPD5 is responsible for addition of the oligo(A) tails. We suggest that deadenylation is coupled to clean 3' end trimming, which might serve to enhance snoRNA stability.

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Language(s): eng - English
 Dates: 2012-03-222012-05
 Publication Status: Published in print
 Pages: -
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 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1261/rna.032292.112
 Degree: -

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Title: RNA-A Publication of the RNA Society
  Other : RNA-Publ. RNA Soc.
Source Genre: Journal
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Publ. Info: New York, NY : Cambridge University Press
Pages: - Volume / Issue: 18 (5) Sequence Number: - Start / End Page: 958 - 972 Identifier: ISSN: 1355-8382
CoNE: https://pure.mpg.de/cone/journals/resource/954925343776