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  Analysis of the methylome of human embryonic stem cells employing methylated DNA immunoprecipitation coupled to next-generation sequencing

Grimm, C., & Adjaye, J. (2012). Analysis of the methylome of human embryonic stem cells employing methylated DNA immunoprecipitation coupled to next-generation sequencing. In K. Turksen (Ed.), Methods in Molecular Biology (pp. 281-295). Humana Press.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-000E-F045-2 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-000E-F046-F
Genre: Book Chapter

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Grimm.pdf (Publisher version), 416KB
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© 2012 Springer Science+Business Media
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 Creators:
Grimm, Christina1, Author              
Adjaye, James2, Author              
Affiliations:
1In vitro Ligand Screening (Zoltán Konthur), Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1479653              
2Molecular Embryology and Aging (James Adjaye), Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1479654              

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Free keywords: DNA Methylation/genetics/*physiology Embryonic Stem Cells/*metabolism High-Throughput Nucleotide Sequencing/*methods Humans Immunoprecipitation/*methods
 Abstract: The analysis of DNA-methylation on a genome-wide scale by next-generation sequencing techniques is an invaluable tool towards the understanding of the epigenetic basis of cellular differentiation. Methylated DNA immunoprecipitation (MeDIP) is an immunocapturing method using an antibody targeting 5-methylcytidine (5 mC) and thereby enriching methylated DNA. MeDIP combined with next-generation sequencing (MeDIP-seq) provides a powerful tool for the analysis of genome-wide DNA-methylation profiles. Here, we describe a protocol for the preparation of MeDIP samples suitable for next-generation sequencing on a Genome Analyser (Illumina).

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Language(s): eng - English
 Dates: 2012
 Publication Status: Published in print
 Pages: -
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 Rev. Method: Peer
 Identifiers: DOI: 10.1007/978-1-61779-794-1_19
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Title: Methods in Molecular Biology
Source Genre: Series
 Creator(s):
Turksen , Kursad, Editor
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Publ. Info: Humana Press
Pages: - Volume / Issue: 873 Sequence Number: - Start / End Page: 281 - 295 Identifier: ISBN: 978-1-61779-794-1