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  Chromatin immunoprecipitation-based analysis of gene regulatory networks operative in human embryonic stem cells

Jung, M., & Adjaye, J. (2012). Chromatin immunoprecipitation-based analysis of gene regulatory networks operative in human embryonic stem cells. In Methods in molecular biology (pp. 269-80). New York: Springer.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-000E-F057-9 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-000E-F058-7
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 Creators:
Jung, Marc1, Author
Adjaye, James1, Author              
Affiliations:
1Molecular Embryology and Aging (James Adjaye), Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society, Ih, ou_1479654              

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Free keywords: Chromatin/genetics/metabolism Chromatin Immunoprecipitation/*methods Embryonic Stem Cells/*metabolism Gene Regulatory Networks/genetics/*physiology Homeodomain Proteins/genetics/metabolism Humans Octamer Transcription Factor-3/genetics/metabolism Oligonucleotide Array Sequence Analysis
 Abstract: Chromatin immunoprecipitation (ChIP) followed by microarray-based (ChIP-Chip) or next-generation sequencing-based (ChIP-Seq) analysis has been established as a powerful and widely used method to investigate DNA-protein interactions relative to a genomic location in vivo. Here, we present a ChIP-Chip protocol, which utilizes an alternative, easier amplification protocol and when using high-quality ChIP-grade antibodies, will generate enough material for hybridization or sequencing with negligible enrichment bias due to amplification.

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Language(s): eng - English
 Dates: 2012
 Publication Status: Published in print
 Pages: 11
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: DOI: 10.1007/978-1-61779-794-1_18
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Title: Methods in molecular biology
Source Genre: Book
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Publ. Info: New York : Springer
Pages: - Volume / Issue: 873 Sequence Number: - Start / End Page: 269 - 80 Identifier: -