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Free keywords:
Chromatin/genetics/metabolism
Chromatin Immunoprecipitation/*methods
Embryonic Stem Cells/*metabolism
Gene Regulatory Networks/genetics/*physiology
Homeodomain Proteins/genetics/metabolism
Humans
Octamer Transcription Factor-3/genetics/metabolism
Oligonucleotide Array Sequence Analysis
Abstract:
Chromatin immunoprecipitation (ChIP) followed by microarray-based (ChIP-Chip) or next-generation sequencing-based (ChIP-Seq) analysis has been established as a powerful and widely used method to investigate DNA-protein interactions relative to a genomic location in vivo. Here, we present a ChIP-Chip protocol, which utilizes an alternative, easier amplification protocol and when using high-quality ChIP-grade antibodies, will generate enough material for hybridization or sequencing with negligible enrichment bias due to amplification.
