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  Characterization of C-strain "Riems" TAV-epitope escape variants obtained through selective antibody pressure in cell culture

Leifer, I., Blome, S., Blohm, U., Konig, P., Kuster, H., Lange, B., et al. (2012). Characterization of C-strain "Riems" TAV-epitope escape variants obtained through selective antibody pressure in cell culture. Veterinary Research, 43(1): 33. doi:10.1186/1297-9716-43-33.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-000E-F060-4 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-000E-F061-2
Genre: Journal Article

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© 2012 Leifer et al; licensee BioMed Central Ltd
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 Creators:
Leifer, I., Author
Blome, S., Author
Blohm, U., Author
Konig, P., Author
Kuster, H., Author
Lange, B.1, Author              
Beer, M., Author
Affiliations:
1Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society, Berlin, Germany, ou_1433550              

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 Abstract: ABSTRACT: Classical swine fever virus (CSFV) C-strain "Riems" escape variants generated under selective antibody pressure with monoclonal antibodies and a peptide-specific antiserum in cell culture were investigated. Candidates with up to three amino acid exchanges in the immunodominant and highly conserved linear TAV-epitope of the E2-glycoprotein, and additional mutations in the envelope proteins ERNS and E1, were characterized both in vitro and in vivo.It was further demonstrated, that intramuscular immunization of weaner pigs with variants selected after a series of passages elicited full protection against lethal CSFV challenge infection. These novel CSFV C-strain variants with exchanges in the TAV-epitope present potential marker vaccine candidates. The DIVA (differentiating infected from vaccinated animals) principle was tested for those variants using commercially available E2 antibody detection ELISA. Moreover, direct virus differentiation is possible using a real-time RT-PCR system specific for the new C-strain virus escape variants or using differential immunofluorescence staining.

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Language(s): eng - English
 Dates: 2012-04-20
 Publication Status: Published online
 Pages: -
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 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1186/1297-9716-43-33
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Title: Veterinary Research
Source Genre: Journal
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Publ. Info: BioMed Central Ltd
Pages: - Volume / Issue: 43 (1) Sequence Number: 33 Start / End Page: - Identifier: -