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  Identification of biomarkers of human skin ageing in both genders. Wnt signalling - a label of skin ageing?

Makrantonaki, E., Brink, T., Zampeli, V., Elewa, R. M., Mlody, B., Hossini, A. M., et al. (2012). Identification of biomarkers of human skin ageing in both genders. Wnt signalling - a label of skin ageing? PLoS One, 7(11), e50393. doi:10.1371/journal.pone.0050393.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-000E-F070-D Version Permalink: http://hdl.handle.net/11858/00-001M-0000-000E-F071-B
Genre: Journal Article

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Makrantonaki, Evegenia1, 2, Author
Brink, Thore3, Author              
Zampeli, Vasilika1, 2, Author
Elewa, Rana Mohsen1, 4, Author
Mlody, Barbara5, Author              
Hossini, Amir M.1, Author
Hermes, Bjoern2, Author
Krause, Ulf5, Author
Knolle, Jürgen5, Author
Abdallah, Marwa4, Author
Adjaye, James5, Author              
Zouboulis, Christos C.1, Author
Affiliations:
1Departments of Dermatology, Venereology, Allergology and Immunology, Dessau Medical Center , Dessau, Germany, ou_persistent22              
2nstitute of Clinical Pharmacology and Toxicology, Charite´ Universitaetsmedizin Berlin, Berlin, Germany, ou_persistent22              
3Department of Vertebrate Genomics, Max Planck Institute for Molecular Genetics, Ihnestr. 63- Berlin, Germany, ou_persistent22              
4Department of Dermatology, Andrology and Venereology, Faculty of Medicine, Ain-Shams University, Hassan Ibrahim Hassan, Nasr City, Cairo, Egypt, ou_persistent22              
5Molecular Embryology and Aging (James Adjaye), Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society, Ihnestr. 63-74, Berlin, Germany, ou_1479654              

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 Abstract: The goal of our work has been to investigate the mechanisms of gender-independent human skin ageing and examine the hypothesis of skin being an adequate model of global ageing. For this purpose, whole genome gene profiling was employed in sun-protected skin obtained from European Caucasian young and elderly females (mean age 26.7+/-4 years [n1 = 7] and 70.75+/-3.3 years [n2 = 4], respectively) and males (mean age 25.8+/-5.2 years [n3 = 6] and 76+/-3.8 years [n4 = 7], respectively) using the Illumina array platform. Confirmation of gene regulation was performed by real-time RT-PCR and immunohistochemistry. 523 genes were significantly regulated in female skin and 401 genes in male skin for the chosen criteria. Of these, 183 genes exhibited increased and 340 decreased expression in females whereas 210 genes showed increased and 191 decreased expression in males with age. In total, 39 genes were common in the target lists of significant regulated genes in males and females. 35 of these genes showed increased (16) or decreased (19) expression independent of gender. Only 4 overlapping genes (OR52N2, F6FR1OP2, TUBAL3 and STK40) showed differential regulation with age. Interestingly, Wnt signalling pathway showed to be significantly downregulated in aged skin with decreased gene and protein expression for males and females, accordingly. In addition, several genes involved in central nervous system (CNS) ageing (f.i. APP, TAU) showed to be expressed in human skin and were significanlty regulated with age. In conclusion, our study provides biomarkers of endogenous human skin ageing in both genders and highlight the role of Wnt signalling in this process. Furthermore, our data give evidence that skin could be used as a good alternative to understand ageing of different tissues such as CNS.

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Language(s): eng - English
 Dates: 2012-10-242011-12-152012-11-30
 Publication Status: Published online
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 Identifiers: DOI: 10.1371/journal.pone.0050393
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Title: PLoS One
Source Genre: Journal
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Publ. Info: San Francisco, CA : Public Library of Science
Pages: - Volume / Issue: 7 (11) Sequence Number: - Start / End Page: e50393 Identifier: ISSN: 1932-6203
CoNE: /journals/resource/1000000000277850