English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Transcriptomic signature of Leishmania infected mice macrophages: a metabolic point of view

Rabhi, I., Rabhi, S., Ben-Othman, R., Rasche, A., Daskalaki, A., Trentin, B., et al. (2012). Transcriptomic signature of Leishmania infected mice macrophages: a metabolic point of view. PLoS Neglected Tropical Diseases, 6(8): e1763. doi:10.1371/journal.pntd.0001763.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-000E-F080-9 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-000E-F081-7
Genre: Journal Article

Files

show Files
hide Files
:
Rabhi.pdf (Publisher version), 887KB
Name:
Rabhi.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
© Rabhi et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
License:
-

Locators

show

Creators

show
hide
 Creators:
Rabhi, I., Author
Rabhi, S., Author
Ben-Othman, R., Author
Rasche, A.1, Author              
Daskalaki, A.2, Author              
Trentin, B., Author
Piquemal, D., Author
Regnault, B., Author
Descoteaux, A., Author
Guizani-Tabbane, L., Author
Affiliations:
1Bioinformatics (Ralf Herwig), Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society, Berlin, Germany, ou_1479648              
2Systems Biology (Christoph Wierling), Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society, Berlin, Germany, ou_1479656              

Content

show
hide
Free keywords: Anaerobiosis Animals Carbohydrate Metabolism *Gene Expression Profiling Leishmania major/*pathogenicity Lipid Metabolism Macrophages/*metabolism/*parasitology Metabolic Networks and Pathways Mice Mice, Inbred BALB C
 Abstract: We analyzed the transcriptional signatures of mouse bone marrow-derived macrophages at different times after infection with promastigotes of the protozoan parasite Leishmania major. Ingenuity Pathway Analysis revealed that the macrophage metabolic pathways including carbohydrate and lipid metabolisms were among the most altered pathways at later time points of infection. Indeed, L. major promastiogtes induced increased mRNA levels of the glucose transporter and almost all of the genes associated with glycolysis and lactate dehydrogenase, suggesting a shift to anaerobic glycolysis. On the other hand, L. major promastigotes enhanced the expression of scavenger receptors involved in the uptake of Low-Density Lipoprotein (LDL), inhibited the expression of genes coding for proteins regulating cholesterol efflux, and induced the synthesis of triacylglycerides. These data suggested that Leishmania infection disturbs cholesterol and triglycerides homeostasis and may lead to cholesterol accumulation and foam cell formation. Using Filipin and Bodipy staining, we showed cholesterol and triglycerides accumulation in infected macrophages. Moreover, Bodipy-positive lipid droplets accumulated in close proximity to parasitophorous vacuoles, suggesting that intracellular L. major may take advantage of these organelles as high-energy substrate sources. While the effect of infection on cholesterol accumulation and lipid droplet formation was independent on parasite development, our data indicate that anaerobic glycolysis is actively induced by L. major during the establishment of infection.

Details

show
hide
Language(s): eng - English
 Dates: 2012-08-212012
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1371/journal.pntd.0001763
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: PLoS Neglected Tropical Diseases
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 6 (8) Sequence Number: e1763 Start / End Page: - Identifier: -