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  Pushing the detection limits: the evanescent field in surface plasmon resonance and analyte-induced folding observation of long human telomeric repeats

Schlachter, C., Lisdat, F., Frohme, M., Erdmann, V. A., Konthur, Z., Lehrach, H., et al. (2012). Pushing the detection limits: the evanescent field in surface plasmon resonance and analyte-induced folding observation of long human telomeric repeats. Biosensors and Bioelectronics, 31(1), 571-574. doi:10.1016/j.bios.2011.11.003.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-000E-F088-A Version Permalink: http://hdl.handle.net/11858/00-001M-0000-000E-F094-E
Genre: Journal Article

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 Creators:
Schlachter, C., Author
Lisdat, F., Author
Frohme, M., Author
Erdmann, V. A., Author
Konthur, Z.1, Author              
Lehrach, H.2, Author              
Glökler, J.2, Author              
Affiliations:
1In vitro Ligand Screening (Zoltán Konthur), Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society, Berlin, Germany, ou_1479653              
2Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society, Berlin, Germany, ou_1433550              

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Free keywords: Biosensing Techniques/*instrumentation Equipment Design Equipment Failure Analysis Humans Molecular Probe Techniques/*instrumentation Refractometry/*instrumentation Repetitive Sequences, Nucleic Acid/*genetics Reproducibility of Results Sensitivity and Specificity Surface Plasmon Resonance/*instrumentation Telomere/*genetics
 Abstract: Conventional analysis of molecular interactions by surface plasmon resonance is achieved by the observation of optical density changes due to analyte binding to the ligand on the surface. Low molecular weight interaction partners are normally not detected. However, if a macromolecule such as DNA can extend beyond the evanescent field and analyte interaction results in a large-scale contraction, then the refractive index changes due to the increasing amount of macromolecules close to the surface. In our proof-of-principle experiment we could observe the direct folding of long, human telomeric repeats induced by the small analyte potassium using surface plasmon resonance spectroscopy. This work demonstrates the feasibility of new evanescent field-based biosensors that can specifically observe small molecule interactions.

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Language(s): eng - English
 Dates: 2012-01
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1016/j.bios.2011.11.003
 Degree: -

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Title: Biosensors and Bioelectronics
  Other : Biosens. Bioelectron.
Source Genre: Journal
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Publ. Info: Elsevier
Pages: - Volume / Issue: 31 (1) Sequence Number: - Start / End Page: 571 - 574 Identifier: ISSN: 0956-5663
CoNE: /journals/resource/954925577034