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  A simple strand-specific RNA-Seq library preparation protocol combining the Illumina TruSeq RNA and the dUTP methods

Sultan, M., Dokel, S., Amstislavskiy, V., Wuttig, D., Sultmann, H., Lehrach, H., et al. (2012). A simple strand-specific RNA-Seq library preparation protocol combining the Illumina TruSeq RNA and the dUTP methods. Biochemical and Biophysical Research Communications, 422(4), 643-646. doi:10.1016/j.bbrc.2012.05.043.

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Item Permalink: https://hdl.handle.net/11858/00-001M-0000-000E-F0A5-8 Version Permalink: https://hdl.handle.net/11858/00-001M-0000-000E-F0A6-6
Genre: Journal Article

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 Creators:
Sultan, M.1, Author           
Dokel, S., Author
Amstislavskiy, V., Author
Wuttig, D., Author
Sultmann, H., Author
Lehrach, H.2, Author           
Yaspo, M. L.1, Author           
Affiliations:
1Human Chromosome 21 (Marie-Laure Yaspo), Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1479652              
2Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433550              

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Free keywords: Deoxyuracil Nucleotides/*chemistry/genetics Gene Expression Profiling/*methods *Gene Library Humans Sequence Analysis, RNA/*methods Transcriptome
 Abstract: Preserving the original RNA orientation information in RNA-Sequencing (RNA-Seq) experiment is essential to the analysis and understanding of the complexity of mammalian transcriptomes. We describe herein a simple, robust, and time-effective protocol for generating strand-specific RNA-seq libraries suited for the Illumina sequencing platform. We modified the Illumina TruSeq RNA sample preparation by implementing the strand specificity feature using the dUTP method. This protocol uses low amounts of starting material and allows a fast processing within two days. It can be easily implemented and requires only few additional reagents to the original Illumina kit.

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Language(s): eng - English
 Dates: 2012-05-152012-06-15
 Publication Status: Issued
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1016/j.bbrc.2012.05.043
 Degree: -

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Title: Biochemical and Biophysical Research Communications
  Other : Biochem. Biophys. Res. Commun.
Source Genre: Journal
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Publ. Info: Orlando, Fla. : Academic Press
Pages: - Volume / Issue: 422 (4) Sequence Number: - Start / End Page: 643 - 646 Identifier: ISSN: 0006-291X
CoNE: https://pure.mpg.de/cone/journals/resource/954922652205_1