English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Warburg effect and translocation-induced genomic instability: two yeast models for cancer cells

Tosato, V., Grüning, N.-M., Breitenbach, M., Arnak, R., Ralser, M., & Bruschi, C. V. (2013). Warburg effect and translocation-induced genomic instability: two yeast models for cancer cells. Frontiers in Oncology, 2: 212. doi:10.3389/fonc.2012.00212.

Item is

Files

show Files
hide Files
:
Tosato.pdf (Publisher version), 877KB
Name:
Tosato.pdf
Description:
-
OA-Status:
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
© 2013 Tosato, Grüning, Breitenbach, Arnak, Ralser and Bruschi. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in other forums, provided the original authors and source are credited and subject to any copyright notices concerning any third-party graphics etc.
License:
-

Locators

show

Creators

show
hide
 Creators:
Tosato, V., Author
Grüning, N.-M.1, Author           
Breitenbach, M., Author
Arnak, R., Author
Ralser, M.1, Author           
Bruschi, C. V., Author
Affiliations:
1Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society, Berlin, Germany, ou_1433550              

Content

show
hide
Free keywords: -
 Abstract: Yeast has been established as an efficient model system to study biological principles underpinning human health. In this review we focus on yeast models covering two aspects of cancer formation and progression (i) the activity of pyruvate kinase (PK), which recapitulates metabolic features of cancer cells, including the Warburg effect, and (ii) chromosome bridge-induced translocation (BIT) mimiking genome instability in cancer. Saccharomyces cerevisiae is an excellent model to study cancer cell metabolism, as exponentially growing yeast cells exhibit many metabolic similarities with rapidly proliferating cancer cells. The metabolic reconfiguration includes an increase in glucose uptake and fermentation, at the expense of respiration and oxidative phosphorylation (the Warburg effect), and involves a broad reconfiguration of nucleotide and amino acid metabolism. Both in yeast and humans, the regulation of this process seems to have a central player, PK, which is up-regulated in cancer, and to occur mostly on a post-transcriptional and post-translational basis. Furthermore, BIT allows to generate selectable translocation-derived recombinants ("translocants"), between any two desired chromosomal locations, in wild-type yeast strains transformed with a linear DNA cassette carrying a selectable marker flanked by two DNA sequences homologous to different chromosomes. Using the BIT system, targeted non-reciprocal translocations in mitosis are easily inducible. An extensive collection of different yeast translocants exhibiting genome instability and aberrant phenotypes similar to cancer cells has been produced and subjected to analysis. In this review, we hence provide an overview upon two yeast cancer models, and extrapolate general principles for mimicking human disease mechanisms in yeast.

Details

show
hide
Language(s): eng - English
 Dates: 2013-01-18
 Publication Status: Issued
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.3389/fonc.2012.00212
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Frontiers in Oncology
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Frontiers Media S.A.
Pages: - Volume / Issue: 2 Sequence Number: 212 Start / End Page: - Identifier: -