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  Identification of mediator complex 26 (Crsp7) gametologs on platypus X1 and Y5 sex chromosomes: a candidate testis-determining gene in monotremes?

Tsend-Ayush, E., Kortschak, R. D., Bernard, P., Lim, S. L., Ryan, J., Rosenkranz, R., et al. (2012). Identification of mediator complex 26 (Crsp7) gametologs on platypus X1 and Y5 sex chromosomes: a candidate testis-determining gene in monotremes? Chromosome Research, 20(1), 127-138. doi:10.1007/s10577-011-9270-z.

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 Creators:
Tsend-Ayush, Enkhjargal, Author
Kortschak, R. Daniel, Author
Bernard, Pascal, Author
Lim, Shu Ly, Author
Ryan, Janelle, Author
Rosenkranz, Ruben1, Author              
Borodina, Tatiana2, Author              
Dohm, Juliane C.1, 3, Author              
Himmelbauer, Heinz1, 3, Author              
Harley, Vincent R., Author
Grützner, Frank, Author
Affiliations:
1Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society, Berlin, Germany, ou_1433550              
2Technology Development(Alexey Soldatov), Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society, Berlin, Germany, ou_1479657              
3Centre for Genomic Regulation (CRG) and UPF, Barcelona, Spain , ou_persistent22              

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Free keywords: Animals Chromosomes, Artificial, Bacterial/genetics Chromosomes, Mammalian/genetics Evolution, Molecular Gene Expression Regulation Genes, Reporter Genes, sry HEK293 Cells Humans Kidney/cytology/metabolism Male Mediator Complex/*genetics Mice Phylogeny Physical Chromosome Mapping Platypus/*genetics SOX9 Transcription Factor/genetics Sex Determination Processes Testis/cytology/*physiology Transfection X Chromosome/*genetics Y Chromosome/*genetics
 Abstract: The basal lineage of monotremes features an extraordinarily complex sex chromosome system which has provided novel insights into the evolution of mammalian sex chromosomes. Recently, sequence information from autosomes, X chromosomes, and XY-shared pseudoautosomal regions has become available. However, no gene has so far been described on any of the Y chromosome-specific regions. We analyzed sequences derived from Y-specific BAC clones to identify genes with potentially male-specific function. Here, we report the identification and characterization of the mediator complex protein gametologs on platypus Y5 (Crspy). We also identified the X-chromosomal copy which unexpectedly maps to X1 (Crspx). Sequence comparison shows extensive divergence between the X and Y copy, but we found no significant positive selection on either gametolog. Expression analysis shows widespread expression of Crspx. Crspy is expressed exclusively in males with particularly strong expression in testis and kidney. Reporter gene assays to investigate whether Crspx/y can act on the recently discovered mouse Sox9 testis-specific enhancer element did reveal a modest effect together with mouse Sox9 + Sf1, but showed overall no significant upregulation of the reporter gene. This is the first report of a differentiated functional male-specific gene on platypus Y chromosomes, providing new insights into sex chromosome evolution and a candidate gene for male-specific function in monotremes.

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Language(s): eng - English
 Dates: 2012-01-042012-01
 Publication Status: Published in print
 Pages: -
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 Rev. Type: Peer
 Identifiers: DOI: 10.1007/s10577-011-9270-z
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Title: Chromosome Research
Source Genre: Journal
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Publ. Info: Oxford : Rapid Science Publishers
Pages: - Volume / Issue: 20 (1) Sequence Number: - Start / End Page: 127 - 138 Identifier: ISSN: 0967-3849
CoNE: https://pure.mpg.de/cone/journals/resource/954925582185