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  Odd-skipped related genes regulate differentiation of embryonic limb mesenchyme and bone marrow mesenchymal stromal cells

Stricker, S., Mathia, S., Haupt, J., Seemann, P., Meier, J., & Mundlos, S. (2012). Odd-skipped related genes regulate differentiation of embryonic limb mesenchyme and bone marrow mesenchymal stromal cells. Stem Cells and Development, 21(4), 623-633. doi:10.1089/scd.2011.0154.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-000E-F27A-9 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-000E-F27B-7
Genre: Journal Article

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 Creators:
Stricker, Sigmar1, 2, Author              
Mathia, Susanne1, Author
Haupt, Julia1, 3, Author
Seemann, Petra1, 3, Author              
Meier, Julia1, Author
Mundlos, Stefan1, 2, 3, Author              
Affiliations:
1Research Group Development & Disease (Head: Stefan Mundlos), Max Planck Institute for Molecular Genetics, Max Planck Society, Berlin, Germany, ou_1433557              
2Institute for Medical and Human Genetics, University Medicine Charité, Berlin, Germany, ou_persistent22              
3Berlin-Brandenburg Centre for Regenerative Therapies BCRT, University Medicine Charité, Berlin, Germany, ou_persistent22              

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Free keywords: Animals Bone Marrow Cells/cytology/ physiology Cell Differentiation/ physiology Cells, Cultured Chick Embryo Chickens Chondrogenesis/physiology Fibroblasts/cytology/physiology Hindlimb/ embryology Mesenchymal Stromal Cells/cytology/ physiology Mesoderm/cytology/ physiology Mice Osteoblasts/cytology/physiology Transcription Factors/ metabolism
 Abstract: The regulation of progenitor cell differentiation to a specific tissue type is one of the fundamental questions of biology. Here, we identify Osr1 and Osr2, 2 closely related genes encoding zinc finger transcription factors, as being strongly expressed in irregular connective tissue (ICT) fibroblasts in the chicken embryo, suitable as a developmental marker. We provide evidence that both Osr1 and Osr2 regulate mesenchymal cell-type differentiation. Both Osr1 and Osr2 can promote the formation of ICT, a cell type of so far unknown molecular specification, while suppressing differentiation of other tissues such as cartilage and tendon from uncommitted progenitors. Conversely, knockdown of either Osr gene alone or in combination reverses this effect, thereby leading to decreased differentiation of ICT fibroblasts and increased chondrogenesis in vitro. This indicates that Osr genes play a pivotal role in ICT fibroblast differentiation. Undifferentiated mesenchymal cells reside in the adult body in the form of mesenchymal stem cells in the bone marrow cavity. Using bone marrow stromal cells (BMSCs) isolated from chicken fetal long bones, we show that Osr1 and Osr2 have an intrinsic role in BMSC differentiation similar to their role in early embryonic development, that is, the enforcement of CT fibroblast differentiation and the repression of other cell types as exemplified here by osteoblast differentiation.

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Language(s): eng - English
 Dates: 2012
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1089/scd.2011.0154
 Degree: -

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Title: Stem Cells and Development
  Other : Stem Cells Dev.
Source Genre: Journal
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Publ. Info: Larchmont, NY : Mary Ann Liebert, Inc.
Pages: - Volume / Issue: 21 (4) Sequence Number: - Start / End Page: 623 - 633 Identifier: ISSN: 1547-3287
CoNE: https://pure.mpg.de/cone/journals/resource/954925275493