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  Design of protein congeners containing beta-cyclopropylalanine

Acevedo-Rocha, C. G., Geiermann, A.-S., Budisa, N., & Merkel, L. (2013). Design of protein congeners containing beta-cyclopropylalanine. Molecular BioSystems, 8(10), 2719-2723. doi:10.1039/C2MB25193K.

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Acevedo-Rocha, Carlos G.1, 2, Autor           
Geiermann, Anna-S3, Autor
Budisa, Nediljko4, Autor
Merkel, Lars4, Autor
Affiliations:
1Research Department Reetz, Max-Planck-Institut für Kohlenforschung, Max Planck Society, Kaiser-Wilhelm-Platz 1, 45470 Mülheim an der Ruhr, DE, ou_1445588              
2Fachbereich Chemie, Phillipps-Universität Marburg, Hans-Meerwein-Straße, 34032 Marburg, Germany , ou_persistent22              
3cLeopold Franzens University, Institute of Organic Chemistry, Center for Chemistry and Biomedicine, Innrain 80/82, A-6020 Innsbruck, Austria , ou_persistent22              
4Berlin Institute of Technology, Department of Chemistry, Biocatalysis Group, Franklinstrasse 29, 10587 Berlin, Germany , ou_persistent22              

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 Zusammenfassung: Abstract: The non-canonical amino acid (ncAA) analogue of methionine (Met), beta-cyclopropylalanine (Cpa), was successfully incorporated into recombinant proteins expressed in Escherichia coli in a residue-specific manner. Proteins substituted in this way are congeners because they derive from the same gene sequence as the parent protein but contain a fraction of ncAAs. We have expressed congeners using parent and mutant gene sequences of various proteins (lipase, annexin A5, enhanced green fluorescent protein, and barstar) and found that Cpa incorporation is highly dependent on the protein sequence composition. These results indicate that the global amino acid composition of proteins might be a crucial parameter that influences the outcome of unnatural translation. In addition, we could also demonstrate that the chemical nature of the second residue could be essential for successful ncAA incorporation.

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 Datum: 2013-10
 Publikationsstatus: Erschienen
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 Art der Begutachtung: Expertenbegutachtung
 Identifikatoren: DOI: 10.1039/C2MB25193K
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Titel: Molecular BioSystems
Genre der Quelle: Zeitschrift
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Ort, Verlag, Ausgabe: -
Seiten: - Band / Heft: 8 (10) Artikelnummer: - Start- / Endseite: 2719 - 2723 Identifikator: -