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  FOX-2 dependent splicing of ataxin-2 transcript is affected by ataxin-1 overexpression

Welzel, F., Kaehler, C., Isau, M., Hallen, L., Lehrach, H., & Krobitsch, S. (2012). FOX-2 dependent splicing of ataxin-2 transcript is affected by ataxin-1 overexpression. PLoS One, 7(5), e37985-e37985. doi:10.1371/journal.pone.0037985.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-000E-F293-F Version Permalink: http://hdl.handle.net/11858/00-001M-0000-000E-F294-D
Genre: Journal Article

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© 2012 Welzel et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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 Creators:
Welzel, Franziska1, Author              
Kaehler, Christian2, Author              
Isau, Melanie3, Author              
Hallen, Linda1, Author              
Lehrach, Hans1, Author              
Krobitsch, Sylvia2, Author              
Affiliations:
1Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433550              
2Neurodegenerative Disorders (Sylvia Krobitsch), Independent Junior Research Groups (OWL), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1479661              
3Cancer Genomics (Michal-Ruth Schweiger), Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1479649              

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Free keywords: Alternative Splicing Cell Nucleus/metabolism Gene Expression Regulation/ genetics HEK293 Cells HeLa Cells Humans Nerve Tissue Proteins/ genetics Nuclear Proteins/ genetics Protein Binding Protein Isoforms/metabolism Protein Transport RNA, Messenger/genetics/metabolism RNA-Binding Proteins/ metabolism Repressor Proteins/ metabolism
 Abstract: Alternative splicing is a fundamental posttranscriptional mechanism for controlling gene expression, and splicing defects have been linked to various human disorders. The splicing factor FOX-2 is part of a main protein interaction hub in a network related to human inherited ataxias, however, its impact remains to be elucidated. Here, we focused on the reported interaction between FOX-2 and ataxin-1, the disease-causing protein in spinocerebellar ataxia type 1. In this line, we further evaluated this interaction by yeast-2-hybrid analyses and co-immunoprecipitation experiments in mammalian cells. Interestingly, we discovered that FOX-2 localization and splicing activity is affected in the presence of nuclear ataxin-1 inclusions. Moreover, we observed that FOX-2 directly interacts with ataxin-2, a protein modulating spinocerebellar ataxia type 1 pathogenesis. Finally, we provide evidence that splicing of pre-mRNA of ataxin-2 depends on FOX-2 activity, since reduction of FOX-2 levels led to increased skipping of exon 18 in ataxin-2 transcripts. Most striking, we observed that ataxin-1 overexpression has an effect on this splicing event as well. Thus, our results demonstrate that FOX-2 is involved in splicing of ataxin-2 transcripts and that this splicing event is altered by overexpression of ataxin-1.

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Language(s): eng - English
 Dates: 2012-05-30
 Publication Status: Published online
 Pages: -
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 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1371/journal.pone.0037985
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Title: PLoS One
Source Genre: Journal
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Publ. Info: San Francisco, CA : Public Library of Science
Pages: - Volume / Issue: 7 (5) Sequence Number: - Start / End Page: e37985 - e37985 Identifier: ISSN: 1932-6203
CoNE: https://pure.mpg.de/cone/journals/resource/1000000000277850