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  Carborhodol: A new hybrid fluorophore obtained by combination of fluorescein and carbopyronine dye cores.

Sednev, M. V., Wurm, C. A., Belov, V. N., & Hell, S. W. (2013). Carborhodol: A new hybrid fluorophore obtained by combination of fluorescein and carbopyronine dye cores. Bioconjugate Chemistry, 24(4), 690-700. doi:10.1021/bc3006732.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-000E-F768-F Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0028-358C-F
Genre: Journal Article

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http://pubs.acs.org/doi/ipdf/10.1021/bc3006732 (Publisher version)
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Sednev, M. V.1, Author              
Wurm, C. A.1, Author              
Belov, V. N.1, Author              
Hell, S. W.1, Author              
Affiliations:
1Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society, ou_578627              

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 Abstract: Asymmetric hybrid fluorophores are built from the structural elements of two (or even more) symmetric dyes and can develop valuable new features which their parents do not possess. A new hybrid carborhodol dye was obtained by the combination of fluorescein and carbopyronine fluorophores. The brightly fluorescent hybrid dye with a linker and reactive group was prepared in 12 steps with overall yield of 1.6%. In aqueous solutions, it has absorption and emission maxima at 586 and 613 nm, respectively. Antibodies labeled with a carborhodol dye possess broad absorption and emission bands so that the effective Stokes shift is increased (compared with small Stokes shifts of the parent dyes) and the fluorescence quantum yield of 39% at a degree of labeling of 5.2. Two samples of secondary antibodies labeled with carborhodol and the benchmark red-emitting rhodamine dye (KK114) were used in two-color imaging experiments with excitation at 514-532 (carborhodol dye) and 633-640 nm (KK114). When emitted light was detected above 650 nm, the novel carborhodol dye provided a lower crosstalk than spectrally similar emitters (e. g., Atto594; crosstalk 40-60% with KK114 under the same conditions). The optical resolution of ca. 80 nm was attained using the new dye in stimulated emission depleted (STED) microscopy. The relatively short fluorescence lifetime in conjugates with antibodies (τ = 1.2-1.6 ns) suggests the possibility of dual FLIM with numerous dyes having τ values in the range of 3-5 ns. All of these features make the carborhodol fluorophore a valuable addition to the family of the red-emitting fluorescent dyes.

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Language(s): eng - English
 Dates: 2013-03-212013-04-17
 Publication Status: Published in print
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 Rev. Method: Peer
 Identifiers: DOI: 10.1021/bc3006732
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Title: Bioconjugate Chemistry
Source Genre: Journal
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Pages: - Volume / Issue: 24 (4) Sequence Number: - Start / End Page: 690 - 700 Identifier: ISSN: 1043-1802