English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Storage and uptake of D-serine into astrocytic synaptic-like vesicles specify gliotransmission.

Martineau, M., Shi, T., Puyal, J., Knolhoff, A. M., Dulong, J., Gasnier, B., et al. (2013). Storage and uptake of D-serine into astrocytic synaptic-like vesicles specify gliotransmission. Journal of Neuroscience, 33(8), 3413-3423. doi:10.1523/JNEUROSCI.3497-12.2013.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-000E-F7B1-7 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0028-1D0E-B
Genre: Journal Article

Files

show Files
hide Files
:
1739300.pdf (Publisher version), 3MB
Name:
1739300.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show
hide
Description:
-

Creators

show
hide
 Creators:
Martineau, M., Author
Shi, T., Author
Puyal, J., Author
Knolhoff, A. M., Author
Dulong, J., Author
Gasnier, B., Author
Klingauf, J., Author
Sweedler, J. V., Author
Jahn, R.1, Author              
Mothet, J. P., Author
Affiliations:
1Department of Neurobiology, MPI for biophysical chemistry, Max Planck Society, ou_578595              

Content

show
hide
Free keywords: -
 Abstract: Glial cells are increasingly recognized as active players that profoundly influence neuronal synaptic transmission by specialized signaling pathways. In particular, astrocytes have been shown recently to release small molecules, such as the amino acids L-glutamate and D-serine as "gliotransmitters," which directly control the efficacy of adjacent synapses. However, it is still controversial whether gliotransmitters are released from a cytosolic pool or by Ca2+-dependent exocytosis from secretory vesicles, i.e., by a mechanism similar to the release of synaptic vesicles in synapses. Here we report that rat cortical astrocytes contain storage vesicles that display morphological and biochemical features similar to neuronal synaptic vesicles. These vesicles share some, but not all, membrane proteins with synaptic vesicles, including the SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) synaptobrevin 2, and contain both L-glutamate and D-serine. Furthermore, they show uptake of L-glutamate and D-serine that is driven by a proton electrochemical gradient. D-Serine uptake is associated with vesicle acidification and is dependent on chloride. Whereas L-serine is not transported, serine racemase, the synthesizing enzyme for D-serine, is anchored to the membrane of the vesicles, allowing local generation of D-serine. Finally, we reveal a previously unexpected mutual vesicular synergy between D-serine and L-glutamate filling in glia vesicles. We conclude that astrocytes contain vesicles capable of storing and releasing D-serine, L-glutamate, and most likely other neuromodulators in an activity-dependent manner.

Details

show
hide
Language(s): eng - English
 Dates: 2013-02-20
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1523/JNEUROSCI.3497-12.2013
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Journal of Neuroscience
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 33 (8) Sequence Number: - Start / End Page: 3413 - 3423 Identifier: -