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  Insulin and insulin like growth factor II endocytosis and signaling via insulin receptor B.

Giudice, J., Barcos, L. S., Guaimas, F. F., Penas-Steinhardt, A., Giordano, L., Jares-Erijman, E. A., et al. (2013). Insulin and insulin like growth factor II endocytosis and signaling via insulin receptor B. Cell Communication and Signaling, 11: 18. doi:10.1186/1478-811X-11-18.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-000E-F98F-7 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0027-C7CC-6
Genre: Journal Article

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 Creators:
Giudice, J., Author
Barcos, L. S., Author
Guaimas, F. F., Author
Penas-Steinhardt, A., Author
Giordano, L.1, Author              
Jares-Erijman, E. A., Author
Leskow, F. C., Author
Affiliations:
1Emeritus Group Laboratory of Cellular Dynamics, MPI for biophysical chemistry, Max Planck Society, ou_578629              

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Free keywords: Insulin / IGF-II, Insulin receptor, Microscopy, Quantum dots, Endocytosis, Signaling
 Abstract: Background: Insulin and insulin-like growth factors (IGFs) act on tetrameric tyrosine kinase receptors controlling essential functions including growth, metabolism, reproduction and longevity. The insulin receptor (IR) binds insulin and IGFs with different affinities triggering different cell responses. Results: We showed that IGF-II induces cell proliferation and gene transcription when IR-B is over-expressed. We combined biotinylated ligands with streptavidin conjugated quantum dots and visible fluorescent proteins to visualize the binding of IGF-II and insulin to IR-B and their ensuing internalization. By confocal microscopy and flow cytometry in living cells, we studied the internalization kinetic through the IR-B of both IGF-II, known to elicit proliferative responses, and insulin, a regulator of metabolism. Conclusions: IGF-II promotes a faster internalization of IR-B than insulin. We propose that IGF-II differentially activates mitogenic responses through endosomes, while insulin-activated IR-B remains at the plasma membrane. This fact could facilitate the interaction with key effector molecules involved in metabolism regulation.

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Language(s): eng - English
 Dates: 2013-03-11
 Publication Status: Published online
 Pages: 15
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 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1186/1478-811X-11-18
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Title: Cell Communication and Signaling
Source Genre: Journal
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Pages: - Volume / Issue: 11 Sequence Number: 18 Start / End Page: - Identifier: -