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  Mitochondrial SIRT4-type proteins in Caenorhabditis elegans and mammals interact with pyruvate carboxylase and other acetylated biotin-dependent carboxylases.

Wirth, M., Karaca, S., Wenzel, D., Ho, L., Tishkoff, D., Lombard, D. B., et al. (2013). Mitochondrial SIRT4-type proteins in Caenorhabditis elegans and mammals interact with pyruvate carboxylase and other acetylated biotin-dependent carboxylases. Mitochondrion, 13(6), 705-720. doi:10.1016/j.mito.2013.02.002.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-000E-FC29-F Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0027-C83D-2
Genre: Journal Article

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 Creators:
Wirth, M.1, Author              
Karaca, S.1, Author              
Wenzel, D.2, Author              
Ho, L., Author
Tishkoff, D., Author
Lombard, D. B., Author
Verdin, E., Author
Urlaub, H.3, Author              
Jedrusik-Bode, M.4, Author              
Fischle, W.1, Author              
Affiliations:
1Research Group of Chromatin Biochemistry, MPI for biophysical chemistry, Max Planck Society, ou_578604              
2Facility for Electron Microscopy, MPI for biophysical chemistry, Max Planck Society, ou_578615              
3Research Group of Bioanalytical Mass Spectrometry, MPI for biophysical chemistry, Max Planck Society, ou_578613              
4Department of Genes and Behavior, MPI for biophysical chemistry, Max Planck Society, ou_persistent34              

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Free keywords: Sirtuins; SIRT4; C. elegans; Biotin-dependent carboxylase; Pyruvate carboxylase; Protein acetylation
 Abstract: The biological and enzymatic function of SIRT4 is largely uncharacterized. We show that the Caenorhabditis elegans SIR-2.2 and SIR-2.3 orthologs of SIRT4 are ubiquitously expressed, also localize to mitochondria and function during oxidative stress. Further, we identified conserved interaction with mitochondrial biotin-dependent carboxylases (PC, PCC, MCCC), key enzymes in anaplerosis and ketone body formation. The carboxylases were found acetylated on multiple lysine residues and detailed analysis of mPC suggested that one of these residues, K748ac, might regulate enzymatic activity. Nevertheless, no changes in mPC acetylation levels and enzymatic activity could be detected upon overexpression or loss of functional SIRT4.

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Language(s): eng - English
 Dates: 2013-02-212013-11
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1016/j.mito.2013.02.002
 Degree: -

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Title: Mitochondrion
Source Genre: Journal
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Publ. Info: -
Pages: - Volume / Issue: 13 (6) Sequence Number: - Start / End Page: 705 - 720 Identifier: -