English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  A fluorescence based method for the quantification of surface functional groups in closed micro- and nanofluidic channels.

Wang, Y., Lowe, R. D., Mejia, Y. X., Feindt, H., Steltenkamp, S., & Burg, T. P. (2013). A fluorescence based method for the quantification of surface functional groups in closed micro- and nanofluidic channels. Biomicrofluidics, 7(2): 026503. doi:10.1063/1.4802270.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-000E-FEC8-A Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0028-E59D-D
Genre: Journal Article

Files

show Files
hide Files
:
1747060.pdf (Publisher version), 2MB
Name:
1747060.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Creators

show
hide
 Creators:
Wang, Y.1, Author              
Lowe, R. D.1, Author              
Mejia, Y. X.1, Author              
Feindt, H., Author
Steltenkamp, S., Author
Burg, T. P.1, Author              
Affiliations:
1Research Group of Biological Micro- and Nanotechnology, MPI for Biophysical Chemistry, Max Planck Society, ou_578602              

Content

show
hide
Free keywords: -
 Abstract: Surface analysis is critical for the validation of microfluidic surface modifications for biology, chemistry, and physics applications. However, until now quantitative analytical methods have mostly been focused on open surfaces. Here, we present a new fluorescence imaging method to directly measure the surface coverage of functional groups inside assembled microchannels over a wide dynamic range. A key advance of our work is the elimination of self-quenching to obtain a linear signal even with a high density of functional groups. This method is applied to image the density and monitor the stability of vapor deposited silane layers in bonded silicon/glass micro- and nanochannels.

Details

show
hide
Language(s): eng - English
 Dates: 2013-04-22
 Publication Status: Published online
 Pages: 11
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1063/1.4802270
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Biomicrofluidics
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 7 (2) Sequence Number: 026503 Start / End Page: - Identifier: -