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  Lateral Membrane Diffusion Modulated by a Minimal Actin Cortex

Heinemann, F., Vogel, S. K., & Schwille, P. (2013). Lateral Membrane Diffusion Modulated by a Minimal Actin Cortex. BIOPHYSICAL JOURNAL, 104(7), 1465-1475. doi:10.1016/j.bpj.2013.02.042.

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 Creators:
Heinemann, Fabian1, Author              
Vogel, Sven K.1, Author              
Schwille, Petra1, Author              
Affiliations:
1Schwille, Petra / Cellular and Molecular Biophysics, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565169              

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Free keywords: FLUORESCENCE CORRELATION SPECTROSCOPY; GIANT UNILAMELLAR VESICLES; SINGLE-MOLECULE TRACKING; UNDERGO HOP DIFFUSION; PLASMA-MEMBRANE; TRANSLATIONAL DIFFUSION; CELL-MEMBRANE; PHASE-SEPARATION; LIPID DIFFUSION; LIVING CELLS
 Abstract: Diffusion of lipids and proteins within the cell membrane is essential for numerous membrane-dependent processes including signaling and molecular interactions. It is assumed that the membrane-associated cytoskeleton modulates lateral diffusion. Here, we use a minimal actin cortex to directly study proposed effects of an actin meshwork on the diffusion in a well-defined system. The lateral diffusion of a lipid and a protein probe at varying densities of membrane-bound actin was characterized by fluorescence correlation spectroscopy (FCS). A clear correlation of actin density and reduction in mobility was observed for both the lipid and the protein probe. At high actin densities, the effect on the protein probe was similar to 3.5-fold stronger compared to the lipid. Moreover, addition of myosin filaments, which contract the actin mesh, allowed switching between fast and slow diffusion in the minimal system. Spot variation FCS was in accordance with a model of fast microscopic diffusion and slower macroscopic diffusion. Complementing Monte Carlo simulations support the analysis of the experimental FCS data. Our results suggest a stronger interaction of the actin mesh with the larger protein probe compared to the lipid. This might point toward a mechanism where cortical actin controls membrane diffusion in a strong size-dependent manner.

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Language(s): eng - English
 Dates: 2013
 Publication Status: Published in print
 Pages: 11
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: ISI: 000317943300013
DOI: 10.1016/j.bpj.2013.02.042
 Degree: -

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Title: BIOPHYSICAL JOURNAL
Source Genre: Journal
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Publ. Info: 600 TECHNOLOGY SQUARE, 5TH FLOOR, CAMBRIDGE, MA 02139 USA : CELL PRESS
Pages: - Volume / Issue: 104 (7) Sequence Number: - Start / End Page: 1465 - 1475 Identifier: ISSN: 0006-3495