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  Micro-optical lens array for fluorescence detection in droplet-based microfluidics.

Lim, J., Gruner, P., Konrad, M., & Baret, J. C. (2013). Micro-optical lens array for fluorescence detection in droplet-based microfluidics. Lab on a Chip, 13(8), 1472-1475. doi:10.1039/C3LC41329B.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0013-7643-6 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0027-CA31-A
Genre: Journal Article

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 Creators:
Lim, Jiseok1, Author              
Gruner, P., Author
Konrad, M.1, Author              
Baret, J. C., Author
Affiliations:
1Research Group of Enzyme Biochemistry, MPI for biophysical chemistry, Max Planck Society, ou_578612              

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 Abstract: We demonstrate the design and integration of droplet-based microfluidic devices with microoptical element arrays for enhanced detection of fluorescent signals. We show that the integration of microlenses and mirror surfaces in these devices results in an 8-fold increase in the fluorescence signal and in improved spatial resolution. Using an array of microlenses, massively parallel detection of droplets containing fluorescent dyes was achieved, leading to detection throughputs of about 2000 droplets per second and per lens, parallelized over 625 measurement points.

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Language(s): eng - English
 Dates: 2013-01-302013
 Publication Status: Published in print
 Pages: -
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 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1039/C3LC41329B
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Title: Lab on a Chip
Source Genre: Journal
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Pages: - Volume / Issue: 13 (8) Sequence Number: - Start / End Page: 1472 - 1475 Identifier: -