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  Molecular profiling of synaptic vesicle docking sites reveals novel proteins but few differences between glutamatergic and GABAergic synapses.

Boyken, J., Grønborg, M., Riedel, D., Urlaub, H., Jahn, R., & Chua, J. J. (2013). Molecular profiling of synaptic vesicle docking sites reveals novel proteins but few differences between glutamatergic and GABAergic synapses. Neuron, 78(2), 285-297. doi:10.1016/j.neuron.2013.02.027.

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 Urheber:
Boyken, J.1, Autor           
Grønborg, M.1, Autor           
Riedel, D.2, Autor           
Urlaub, H.3, Autor           
Jahn, R.1, Autor           
Chua, J. J.4, Autor           
Affiliations:
1Department of Neurobiology, MPI for biophysical chemistry, Max Planck Society, ou_578595              
2Facility for Electron Microscopy, MPI for biophysical chemistry, Max Planck Society, ou_578615              
3Research Group of Bioanalytical Mass Spectrometry, MPI for biophysical chemistry, Max Planck Society, ou_578613              
4Research Group of Protein Trafficking in Synaptic Development and Function, MPI for Biophysical Chemistry, Max Planck Society, ou_1933287              

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 Zusammenfassung: Neurotransmission involves calcium-triggered fusion of docked synaptic vesicles at specialized presynaptic release sites. While many of the participating proteins have been identified, the molecular composition of these sites has not been characterized comprehensively. Here, we report a procedure to biochemically isolate fractions highly enriched in docked synaptic vesicles. The fraction is largely free of postsynaptic proteins and most other organelles while containing most known synaptic vesicle and active zone proteins. Numerous presynaptic transmembrane proteins were also identified, together with over 30 uncharacterized proteins, many of which are evolutionarily conserved. Quantitative proteomic comparison of glutamate- and GABA-specific docking complexes revealed that, except of neurotransmitter-specific enzymes and transporters, only few proteins were selectively enriched in either fraction. We conclude that the core machinery involved in vesicle docking and exocytosis does not show compositional differences between the two types of synapses.

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Sprache(n): eng - English
 Datum: 2013-04-24
 Publikationsstatus: Erschienen
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 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: Expertenbegutachtung
 Identifikatoren: DOI: 10.1016/j.neuron.2013.02.027
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Titel: Neuron
Genre der Quelle: Zeitschrift
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Seiten: - Band / Heft: 78 (2) Artikelnummer: - Start- / Endseite: 285 - 297 Identifikator: -