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  Molecular profiling of synaptic vesicle docking sites reveals novel proteins but few differences between glutamatergic and GABAergic synapses.

Boyken, J., Grønborg, M., Riedel, D., Urlaub, H., Jahn, R., & Chua, J. J. (2013). Molecular profiling of synaptic vesicle docking sites reveals novel proteins but few differences between glutamatergic and GABAergic synapses. Neuron, 78(2), 285-297. doi:10.1016/j.neuron.2013.02.027.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0013-76AF-3 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0028-1F6B-7
Genre: Journal Article

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 Creators:
Boyken, J.1, Author              
Grønborg, M.1, Author              
Riedel, D.2, Author              
Urlaub, H.3, Author              
Jahn, R.1, Author              
Chua, J. J.4, Author              
Affiliations:
1Department of Neurobiology, MPI for biophysical chemistry, Max Planck Society, ou_578595              
2Facility for Electron Microscopy, MPI for biophysical chemistry, Max Planck Society, ou_578615              
3Research Group of Bioanalytical Mass Spectrometry, MPI for biophysical chemistry, Max Planck Society, ou_578613              
4Research Group of Protein Trafficking in Synaptic Development and Function, MPI for Biophysical Chemistry, Max Planck Society, ou_1933287              

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 Abstract: Neurotransmission involves calcium-triggered fusion of docked synaptic vesicles at specialized presynaptic release sites. While many of the participating proteins have been identified, the molecular composition of these sites has not been characterized comprehensively. Here, we report a procedure to biochemically isolate fractions highly enriched in docked synaptic vesicles. The fraction is largely free of postsynaptic proteins and most other organelles while containing most known synaptic vesicle and active zone proteins. Numerous presynaptic transmembrane proteins were also identified, together with over 30 uncharacterized proteins, many of which are evolutionarily conserved. Quantitative proteomic comparison of glutamate- and GABA-specific docking complexes revealed that, except of neurotransmitter-specific enzymes and transporters, only few proteins were selectively enriched in either fraction. We conclude that the core machinery involved in vesicle docking and exocytosis does not show compositional differences between the two types of synapses.

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Language(s): eng - English
 Dates: 2013-04-24
 Publication Status: Published in print
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 Rev. Method: Peer
 Identifiers: DOI: 10.1016/j.neuron.2013.02.027
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Title: Neuron
Source Genre: Journal
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Pages: - Volume / Issue: 78 (2) Sequence Number: - Start / End Page: 285 - 297 Identifier: -