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  Photoswitching emission with rhodamine spiroamides for super-resolution fluorescence nanoscopies.

Belov, V. N., & Bossi, M. L. (2013). Photoswitching emission with rhodamine spiroamides for super-resolution fluorescence nanoscopies. Israel Journal of Chemistry, 53(5), 267-279. doi:10.1002/ijch.201300017.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0013-7887-B Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0027-CDEF-6
Genre: Journal Article

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1752634.pdf (Publisher version), 3MB
 
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 Creators:
Belov, V. N.1, Author              
Bossi, M. L.1, Author              
Affiliations:
1Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society, ou_578627              

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Free keywords: fluorescence; optical nanoscopy; photochemistry; photochromism
 Abstract: Spatial resolution in far-field fluorescence microscopy is limited by diffraction to about 200 nm. With the aid of photoswitchable fluorophores, the diffraction barrier has been successfully overcome, allowing unprecedented resolution in the order of single biomolecules. The imaging process demands markers with strict and reliable control of the switching, to keep most of the markers in a non-emissive state most of the time and to bring a tiny number back to an emissive state, and detection at the single-molecule level. Herein, we describe the use of rhodamine spiroamides with unique photophysical properties as molecular probes for super-resolution techniques based on the localization of single emitters. This family of photochromic and fluorescent compounds fulfils the stringent requirements for such imaging methods; these compounds are robust and capable of enduring single-molecule detection in diverse environments. This has allowed meaningful images with resolution down to a few nanometres. Their design, synthesis and implementation is discussed along with imaging applications in material and life sciences.

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Language(s): eng - English
 Dates: 2013-05-092013-05
 Publication Status: Published in print
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 Rev. Method: Peer
 Identifiers: DOI: 10.1002/ijch.201300017
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Title: Israel Journal of Chemistry
Source Genre: Journal
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Pages: - Volume / Issue: 53 (5) Sequence Number: - Start / End Page: 267 - 279 Identifier: -