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  Direct interaction of the major light-harvesting complex II and PsbS in nonphotochemical quenching.

Wilk, L., Grunwald, M., Liao, P. N., Walla, P. J., & Kühlbrandt, W. (2013). Direct interaction of the major light-harvesting complex II and PsbS in nonphotochemical quenching. Proceedings of the National Academy of Sciences of the United States of America, 110(14), 5452-5456. doi:10.1073/pnas.1205561110.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0013-78B1-D Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0028-811F-6
Genre: Journal Article

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Wilk, L., Author
Grunwald, M.1, Author              
Liao, P. N., Author
Walla, P. J.1, Author              
Kühlbrandt, W., Author
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1Research Group of Biomolecular Spectroscopy and Single-Molecule Detection, MPI for biophysical chemistry, Max Planck Society, ou_578565              

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 Abstract: The photosystem II (PSII) subunit S (PsbS) plays a key role in non-photochemical quenching, a photoprotective mechanism for dissipation of excess excitation energy in plants. The precise function of PsbS in nonphotochemical quenching is unknown. By reconstituting PsbS together with the major light-harvesting complex of PSII (LHC-II) and the xanthophyll zeaxanthin (Zea) into proteoliposomes, we have tested the individual contributions of PSII complexes and Zea to chlorophyll (Chl) fluorescence quenching in a membrane environment. We demonstrate that PsbS is stable in the absence of pigments in vitro. Significant Chl fluorescence quenching of reconstituted LHC-II was observed in the presence of PsbS and Zea, although neither Zea nor PsbS alone was sufficient to induce the same quenching. Coreconstitution with PsbS resulted in the formation of LHC-II/PsbS heterodimers, indicating their direct interaction in the lipid bilayer. Two-photon excitation measurements on liposomes containing LHC-II, PsbS, and Zea showed an increase of electronic interactions between carotenoid S-1 and Chl states, Phi(CarS1-Chl)(Coupling), that correlated directly with Chl fluorescence quenching. These findings are in agreement with a carotenoid-dependent Chl fluorescence quenching by direct interactions of LHCs of PSII with PsbS monomers.

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Language(s): eng - English
 Dates: 2013-03-182013-04-02
 Publication Status: Published in print
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 Rev. Method: Peer
 Identifiers: DOI: 10.1073/pnas.1205561110
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Title: Proceedings of the National Academy of Sciences of the United States of America
Source Genre: Journal
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Pages: - Volume / Issue: 110 (14) Sequence Number: - Start / End Page: 5452 - 5456 Identifier: -