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  Efficient CO-CA transfer in highly deuterated proteins by band-selective homonuclear cross-polarization.

Chevelkov, V., Giller, K., Becker, S., & Lange, A. (2013). Efficient CO-CA transfer in highly deuterated proteins by band-selective homonuclear cross-polarization. Journal of Magnetic Resonance, 230, 205-211. doi:10.1016/j.jmr.2013.02.021.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0013-78D0-7 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0028-EEFF-B
Genre: Journal Article

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 Creators:
Chevelkov, V.1, Author              
Giller, K.2, Author              
Becker, S.2, Author              
Lange, A.1, Author              
Affiliations:
1Research Group of Solid-State NMR, MPI for Biophysical Chemistry, Max Planck Society, ou_persistent35              
2Department of NMR-Based Structural Biology, MPI for biophysical chemistry, Max Planck Society, ou_578567              

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 Abstract: Robust and efficient band-selective magnetization transfer between CO and CA spins can be achieved in highly deuterated solid proteins by dipolar-based homonuclear cross polarization. The approach is designed for moderate magic-angle spinning rates and high external magnetic fields where the isotropic chemical shift difference of CO and CA considerably exceeds the spinning rate. The most efficient recoupling is achieved when the sum of effective radio-frequency fields on CO and CA resonances equals two times the spinning rate. This method can be directly implemented in proton-detected versions of inter-residual correlation experiments as needed for resonance assignment in protein solid-state NMR spectroscopy.

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Language(s): eng - English
 Dates: 2013-03-152013-05
 Publication Status: Published in print
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 Rev. Method: Peer
 Identifiers: DOI: 10.1016/j.jmr.2013.02.021
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Title: Journal of Magnetic Resonance
Source Genre: Journal
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Pages: - Volume / Issue: 230 Sequence Number: - Start / End Page: 205 - 211 Identifier: -