English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  CAP, a new human suspension cell line for influenza virus production

Genzel, Y., Behrendt, I., Rödig, J., Rapp, E., Kueppers, C., Kochanek, S., et al. (2013). CAP, a new human suspension cell line for influenza virus production. Applied Microbiology and Biotechnology, 97(1), 111-122. doi:10.1007/s00253-012-4238-2.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0013-87D0-C Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-A01A-D
Genre: Journal Article

Files

show Files

Locators

show

Creators

show
hide
 Creators:
Genzel, Yvonne1, Author              
Behrendt, Ilona1, Author              
Rödig, Jana1, Author              
Rapp, Erdmann1, Author              
Kueppers, Claudia2, Author
Kochanek, Stefan2, Author
Schiedner, Gudrun2, Author
Reichl, Udo1, 3, Author              
Affiliations:
1Bioprocess Engineering, Max Planck Institute for Dynamics of Complex Technical Systems, Max Planck Society, ou_1738140              
2University of UmDepartment of Gene TherapyUlm, GermanyCEVEC Pharmaceuticals GmbHCologne, GermanyOtto-von-Guericke-UniversätitMagdeburg, Germany, ou_persistent22              
3Otto-von-Guericke-Universität Magdeburg, ou_1738156              

Content

show
hide
Free keywords: -
 Abstract: Forced by major drawbacks of egg-based influenza virus production, several studies focused on the establishment and optimization of cell-based production systems. Among numerous possible host cell lines from duck, monkey, canine, chicken, mouse and human origin only a few will meet regulatory requirements, accomplish industrial standards and result in high virus titers. From primary virus isolation up to large scale manufacturing of human vaccines, however, the most logical choice seems to be the use of human cell lines. For this reason, we evaluated the recently established CAP cell line derived from human amniocytes for its potential in influenza virus production in suspension culture in small scale shaker flask and stirred tank bioreactor experiments. Different human and animal influenza viruses could be adapted to produce hemagglutination (HA) titers of at least 2.0 log10 HA units/100 µL without further process optimization. Adjusting trypsin activity as well as infection conditions (multiplicity of infection, infection medium) resulted in HA titers of up to 3.2 log10 HA units/100 µL and maximum cell-specific virus productivities of 6400 virions/cell (for human influenza A/PR/8/34 as a reference). Surface membrane expression of sialyloligosaccharides as well as HA N-glycosylation patterns were characterized. Overall, experimental results clearly demonstrate the potential of CAP cells for achieving high virus yields for different influenza strains, and the option to introduce a highly attractive fully characterized human cell line compliant with regulatory and industrial requirements as an alternative for influenza virus vaccine production. COPYRIGHT © Springer, Part of Springer Science+Business Media [accessed November 2nd 2012]

Details

show
hide
Language(s): eng - English
 Dates: 2013
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Applied Microbiology and Biotechnology
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 97 (1) Sequence Number: - Start / End Page: 111 - 122 Identifier: -