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  Metabolic Flux Analysis of AAT Production in AGE1.HN.AAT Cells under Different Cultivation Conditions

Freund, S., Rath, A., Rose, T., Sandig, V., & Reichl, U. (2012). Metabolic Flux Analysis of AAT Production in AGE1.HN.AAT Cells under Different Cultivation Conditions. Poster presented at ESBES - 9 th European Symposium on Biochemical Engineering Science, Istanbul.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0013-887A-8 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0025-1A72-6
Genre: Poster

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 Creators:
Freund, Susann1, 2, Author              
Rath, Alexander1, Author              
Rose, Thomas, Author
Sandig, Volker, Author
Reichl, Udo1, 3, Author              
Affiliations:
1Bioprocess Engineering, Max Planck Institute for Dynamics of Complex Technical Systems, Max Planck Society, ou_1738140              
2International Max Planck Research School (IMPRS), Max Planck Institute for Dynamics of Complex Technical Systems, Max Planck Society, ou_1738143              
3Otto-von-Guericke-Universität Magdeburg, ou_1738156              

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 Abstract: Mammalian cells play a major role in pharmaceutical industry and are essential for the production of glycosylated proteins. For industrial application productivity is of main interest. In this study the productivity of the human cell line AGE1.HN.AAT[1] under different experimental conditions is investigated. Cells were cultivated in shaker flasks for 70 h using standard cultivation conditions at 37°C and an initial glucose concentration of 30 mM. In the late exponential growth phase cultivation conditions were changed. As described in literature reduction of temperature resulted in higher product titers. In the absence of glucose, the cell concentration remained constant for about 30 h at a high viability. Interestingly, the cells still produced AAT. Experimental findings will be discussed based on a comprehensive model using metabolic flux analysis.

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Language(s): eng - English
 Dates: 2012
 Publication Status: Not specified
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: -
 Identifiers: eDoc: 635006
 Degree: -

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Title: ESBES - 9 th European Symposium on Biochemical Engineering Science
Place of Event: Istanbul
Start-/End Date: 2012-09-24 - 2012-09-26

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