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  The major soyabean allergen P34 resists proteolysis in vitro and is transported through intestinal epithelial cells by a caveolae-mediated mechanism

Sewekow, E., Bimczok, D., Kähne, T., Faber-Zuschratter, H., Keßler, L. C., Seidel-Morgenstern, A., et al. (2012). The major soyabean allergen P34 resists proteolysis in vitro and is transported through intestinal epithelial cells by a caveolae-mediated mechanism. British Journal of Nutrition, 108(9), 1603-1611. doi:10.1017/S0007114511007045.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0013-8A2A-C Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-B395-F
Genre: Journal Article

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 Creators:
Sewekow, Eva1, Author
Bimczok, Diane1, Author
Kähne, Thilo1, Author
Faber-Zuschratter, Heidi1, Author
Keßler, Lars Christian2, Author              
Seidel-Morgenstern, Andreas1, 2, Author              
Rothkötter, Hermann-Josef1, Author
Affiliations:
1Otto-von-Guericke-Universität Magdeburg, External Organizations, ou_1738156              
2Physical and Chemical Foundations of Process Engineering, Max Planck Institute for Dynamics of Complex Technical Systems, Max Planck Society, ou_1738150              

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 Abstract: Soya is considered to be one of the eight most significant food allergens. Among the allergenic soya proteins determined to date, P34 has been identified as one of the immunodominant soya antigens. Sensitisation to a specific food antigen like P34 generally follows the transit of intact antigens across the intestinal barrier and usually occurs in infants, who are most susceptible to food allergies. In the present study, we used the intestinal epithelial cell line IPEC-J2, which was originally derived from the jejunum of a neonatal piglet, to recapitulate the infant intestinal epithelium and study the binding and uptake of P34 protein. P34 was partially resistant to degradation in an in vitro proteolysis assay. IPEC-J2 cells were able to endocytose intact P34, as shown by immunofluorescence and immunoelectronmicroscopy methods. P34 associated with lipid raft microdomains of IPEC-J2 cells, and disruption of caveolae/lipid raft microdomains using methyl-β-cyclodextrin abolished P34 endocytosis, indicating that the observed endocytosis was mediated by caveolae. Using IPEC-J2 cells grown on Transwell filters, we further demonstrated that P34 is transported through the epithelial monolayer by transcytosis. Piglets frequently show hypersensitivity to soya antigens, and in this study, we show that healthy adult pigs with dietary exposure to soya protein mount an antibody response to soyabean protein P34, suggesting that this protein has entered the body, probably through gastrointestinal uptake. In summary, our data suggest that soya P34 resists proteolysis in the gastrointestinal tract and is transported through the intestinal epithelial barrier, thereby allowing sensitisation of immune cells in the sub-epithelial compartment. © Cambridge University Press 2012 [accessed November 30th 2012]

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Language(s): eng - English
 Dates: 2012
 Publication Status: Published in print
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 Rev. Type: Peer
 Identifiers: eDoc: 611831
DOI: 10.1017/S0007114511007045
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Title: British Journal of Nutrition
Source Genre: Journal
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Pages: - Volume / Issue: 108 (9) Sequence Number: - Start / End Page: 1603 - 1611 Identifier: -